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Metalloproteinases, vascular endothelial growth factor, and angiopoietin 1 and 2 in eutopic and ectopic endometrium Costantino Di Carlo, M.D., Marika Bonifacio, M.D., Giovanni A. Tommaselli, Ph.D., M.D., Giuseppe Bifulco, M.D., Germano Guerra, Ph.D., M.D., Carmine Nappi, M.D. Fertility and Sterility Volume 91, Issue 6, Pages (June 2009) DOI: /j.fertnstert Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions
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Figure 1 (A) Immunostaining for vascular endothelial growth factor (VEGF) in cysts of woman from the study group: (a) stromatic cells and fibers, and (b) venous wall cells. (B) Immunostaining for VEGF in endometrium of woman from the study group: (a) stromatic cells and fibers. (C) Immunostaining for VEGF in endometrium of woman from the control group: (a) stromatic cells and fibers. (A–D: ABC/HRP method, original magnification ×200. A computerized analysis of the image was performed to quantify the expression of the target protein.) (D) Representative gels of RT-PCR for (a) VEGF and (b) glyceraldehydes phosphate dehydrogenase (GAPDH): specimens from cyst of a woman from the study group (1), specimens from the endometrium of a woman from the study group (2), and specimens from the control group (3). Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions
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Figure 2 (A) Immunostaining for angiopoietin 1 (ANGPT1) from cyst of woman from the study group: (a) stromatic fibers and (b) stromatic cells. (B) Immunostaining for ANGPT1 in endometrium of woman from the study group: (a) stromatic cells. (C) Immunostaining for ANGPT 1 in endometrium of woman from the control group: (a) stromatic cells. (A–D: ABC/HRP method, original magnification ×200. A computerized analysis of the image was performed to quantify the expression of the target protein.) (D) Representative gels of RT-PCR for (a) ANGPT 1 and (b) glyceraldehydes phosphate dehydrogenase (GAPDH): specimens from cyst of woman from the study group (1), from endometrium of woman from the study group (2), and from woman from control group (3). Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions
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Figure 3 (A) Immunostaining for MMP-1 (metalloproteinase 1) in cyst of woman from the study group: (a) stromatic cells and fibers. (B) Immunostaining for MMP-1 in endometrium of woman from the study group: (a) stromatic cells and fibers, and (b) venous vessel. (C) Immunostaining for MMP-1 in endometrium of woman from the control group: (a) stromatic cells and fibers. (A–D: ABC/HRP method, original magnification ×200. A computerized analysis of the image was performed to quantify the expression of the target protein.) (D) Representative gels of RT-PCR for (a) MMP-1 and (b) glyceraldehydes phosphate dehydrogenase (GAPDH): specimens from cyst of woman from the study group (1), from endometrium of woman from the study group (2), and from woman from the control group (3). Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions
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Figure 4 (A) Immunohistochemistry data. (B) Semiquantitative RT-PCR values. ∗P<.01 versus endometrium (study group). §P<.05 versus endometrium (study group). Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions
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