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Human Treg cells express PlexinB1.
Human Treg cells express PlexinB1. Human PBMCs were obtained from healthy donors and stained with specific Abs for the indicated cell surface and intracellular markers. Dead cells were eliminated from analysis based on fixable dye exclusion. (A) Human CD3+ cells express Plexin B1 but not NRP-1. (B) Representative dot and density plots are labeled with the gating strategies. Histogram of PlexinB1 staining for CD4+CD25highFoxp3+ cells is shown. Marker-specific staining is shown by the black line with a transparent background, whereas an isotype control Ab staining is shown in solid gray color. Data are representative from three different experiments. Svetlana P. Chapoval et al. ImmunoHorizons 2019;3:71-87 Copyright © 2019 by The Authors
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