1. ADAMTS-1 and ADAMTS-4 Levels Are Elevated in Thoracic Aortic Aneurysms and Dissections 
Pingping Ren, MD, Lin Zhang, MS, Gaiping Xu, MD, Laura C. Palmero, MPH, Paul T. Albini, MD, Joseph S. Coselli, MD, Ying H. Shen, MD, PhD, Scott A. LeMaire, MD 
The Annals of Thoracic Surgery 
Volume 95, Issue 2, Pages (February 2013)
DOI: /j.athoracsur
Copyright © 2013 The Society of Thoracic Surgeons Terms and Conditions

2. Fig 1
Increased expression of ADAMTS-1 and ADAMTS-4 in aneurysm and dissection tissues. (A) Western blot and quantitative analysis of ADAMTS-1 and ADAMTS-4 proteins in control, thoracic aortic aneurysm (TAA), and thoracic aortic dissection (TAD) tissues. (B) Quantitative real-time polymerase chain reaction analysis of ADAMTS-1 and ADAMTS-4 mRNA levels in control, thoracic aortic aneurysm, and thoracic aortic dissection tissues.
The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur )
Copyright © 2013 The Society of Thoracic Surgeons Terms and Conditions

3. Fig 2
Increased abundance of ADAMTS-1 and ADAMTS-4 protein in the media and adventitia of aortic aneurysm and dissection tissues. Representative immunohistochemical staining of ADAMTS-1 (A) and ADAMTS-4 (B) in the media and adventitia of the aortic wall in control, thoracic aortic aneurysm (TAA), and thoracic aortic dissection (TAD) tissues (original magnification ×400).
The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur )
Copyright © 2013 The Society of Thoracic Surgeons Terms and Conditions

4. Fig 3
Localization of ADAMTS-1 and ADAMTS-4 protein in vascular smooth muscle cells and macrophages in aneurysm and dissection tissues. Double immunofluorescence staining showing (A, B) colocalization of ADAMTS-1 and ADAMTS-4 with smooth muscle cell marker SM22 alpha in the medial layer of the aortic wall in thoracic aortic aneurysm (TAA) tissue and (C, D) colocalization of ADAMTS-1 and ADAMTS-4 with macrophage marker CD68 in a macrophage-rich area in adventitia of thoracic aortic aneurysm tissue (original magnification ×400). Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI, blue).
The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur )
Copyright © 2013 The Society of Thoracic Surgeons Terms and Conditions

5. Fig 4
Increased degradation of versican in aortic aneurysm and dissection tissues. (A) Western blot and quantitative analysis of versican degradation products in control, thoracic aortic aneurysm (TAA), and thoracic aortic dissection (TAD) tissues. (B) Correlation between versican degradation and levels of ADAMTS-1 and ADAMTS-4 protein. (C) Quantitative real-time polymerase chain reaction analysis of versican V0 mRNA levels in control, thoracic aortic aneurysm, and thoracic aortic dissection tissues.
The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur )
Copyright © 2013 The Society of Thoracic Surgeons Terms and Conditions

6. Fig 5
Involvement of ADAMTS-4 in transforming growth factor (TGF)-β–induced macrophage migration. (A) Western blot analysis of ADAMTS-4 in macrophages treated with hydrogen peroxide (H2O2), tumor necrosis factor-α (TNF-α), lipopolysaccharide (LPS), TGF-β, or angiotensin II (AngII). (B) Macrophages were transfected with scramble or ADAMTS-4 siRNA before treatment with 10 ng/mL of TGF-β for 24 hours (original magnification ×100). Migration of the treated cells was analyzed by using a transwell assay.
The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur )
Copyright © 2013 The Society of Thoracic Surgeons Terms and Conditions