1. Single human sperm cryopreservation method using hollow-core agarose capsules 
Yasuyuki Araki, Ph.D., Tatsuma Yao, M.S., Yuta Asayama, M.S., Akio Matsuhisa, Ph.D., Yasuhisa Araki, Ph.D. 
Fertility and Sterility 
Volume 104, Issue 4, Pages (October 2015)
DOI: /j.fertnstert
Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Sperm injection into an agarose capsule. (A) Agarose capsule held by a holding pipette before injection. (B) Injection pipette inserted into an agarose capsule. A single sperm was injected into the capsule. (C) Agarose capsules containing a sperm before freezing. (D) Agarose capsules after thawing.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
Devices for freezing capsules. (A–C) A polycarbonate sheet fixed on plastic straw. (D–F) A mesh sheet fixed on plastic straw. (B, C, E, F) Cryoprotectant solution contained capsules on the top of the sheets. (G) The special tool was made using a styrene foam board. It had a square 2.5-cm hole on the top of a styrene foam box. (H) The box floated on liquid nitrogen vapor within a bigger box for freezing the capsules.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions