1. Restriction fragment mass polymorphism (RFMP) analysis based on MALDI-TOF mass spectrometry for detecting antiretroviral resistance in HIV-1 infected patients 
J.-H. Lee, A. Hachiya, S.-K. Shin, J. Lee, H. Gatanaga, S. Oka, K.A. Kirby, Y.T. Ong, S.G. Sarafianos, W.R. Folk, W. Yoo, S.P. Hong, S.- O. Kim 
Clinical Microbiology and Infection 
Volume 19, Issue 6, Pages E263-E270 (June 2013)
DOI: /
Copyright © 2013 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

2. Fig. 1
Evaluation of the sensitivity of the RFMP assay for detection of minor amounts of virus with a defined mixture of K103N. The MALDI-TOF MS spectra and direct sequencing chromatograms shown are representative of experiments repeated three times using mixed populations of wild-type (K103) and NNRTI mutant (N103). The wild-type plasmids were mixed with mutant type at various ratios as follows: (a) 100%, (b) 50%, (c) 20%, (d) 10%, (e) 5% and (f) 1%. Molecular masses of and correspond to N103 and K103, respectively. AI is absolute intensity; m/z is mass-to-charge ratio.
Clinical Microbiology and Infection  , E263-E270DOI: ( / )
Copyright © 2013 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

3. Fig. 2
Comparison of the RFMP and direct sequencing assays for detection of mixed genotypes. Sera were taken from patients infected with HIV-1 carrying ARV drug-resistant mutations and examined by the RFMP and sequencing assays. (a) For codon 103, molecular masses of / and / represent Lys (AAA) and Asn (AAC), respectively. (b) For codon 46, molecular masses of / and / represent Met (ATG) and Ile (ATA), respectively. (c) For codon 215, molecular masses of / and / represent Thr (ACC) and Tyr (TAC), respectively. Each codon was indicated by a red box in the sequencing chromatogram. AI is absolute intensity; m/z is mass-to-charge ratio.
Clinical Microbiology and Infection  , E263-E270DOI: ( / )
Copyright © 2013 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions