1. Cellular localization of the chimeric Iff5-Iff1C, Iff5-Iff2C, Iff5-Iff4C, Iff5-Iff7C, and Iff5-Iff10C proteins.
Cellular localization of the chimeric Iff5-Iff1C, Iff5-Iff2C, Iff5-Iff4C, Iff5-Iff7C, and Iff5-Iff10C proteins. (A) Western blot analysis of the SDS- and βME-solubilized proteins from the cell wall fraction (C1,000) (lanes 1) and of extracted proteins after incubation of the resulting cell walls either in Na-acetate (NaAc) buffer alone (lanes 2) or in NaAc buffer plus 2 U of β-1,6-glucanase (lanes 3) from the V5 epitope-tagged Iff5-1C, -2C, -4C, -7C, and -10C recombinant strains. Samples were separated by SDS-PAGE, transferred onto a nitrocellulose membrane, and immunoblotted with monoclonal anti-V5 antibody. (B) Western blotting with monoclonal anti-V5 antibody of the low-speed supernatant (S1,000) (lane 1), of the SDS-solubilized proteins from the high-speed pellet (C100,000) (lane 2), and of the SDS- and βME-solubilized proteins from the cell wall fraction (C1,000) (lane 3) from the V5 epitope-tagged Iff5-10C strain.
Anita Boisramé et al. Eukaryotic Cell 2011; doi: /EC