1. Volume 57, Issue 6, Pages 2375-2385 (June 2000)
Ischemic acute renal failure: Long-term histology of cell and matrix changes in the rat 
Josephine M. Forbes, Timothy D. Hewitson, Gavin J. Becker, Colin L. Jones 
Kidney International 
Volume 57, Issue 6, Pages (June 2000)
DOI: /j x
Copyright © 2000 International Society of Nephrology Terms and Conditions

2. Figure 1
Morphometric quantitation of inflammatory parameters. (A) Interstitial area. (B) Mo/Mϕ (ED-1). (C) Myofibroblasts (α-SMA). (D) Tubulointerstitial collagen IV. (E) Tubulointerstitial collagen III. *P < 0.05 as compared with 0 day nonischemic control group. #P < 0.05 for ischemic groups as compared with time-matched nonischemic groups. SEM constitutes error bars on all graphs. Where error bars are not evident, the number was too small to be represented on these axes.
Kidney International  , DOI: ( /j x)
Copyright © 2000 International Society of Nephrology Terms and Conditions

3. Figure 1
Morphometric quantitation of inflammatory parameters. (A) Interstitial area. (B) Mo/Mϕ (ED-1). (C) Myofibroblasts (α-SMA). (D) Tubulointerstitial collagen IV. (E) Tubulointerstitial collagen III. *P < 0.05 as compared with 0 day nonischemic control group. #P < 0.05 for ischemic groups as compared with time-matched nonischemic groups. SEM constitutes error bars on all graphs. Where error bars are not evident, the number was too small to be represented on these axes.
Kidney International  , DOI: ( /j x)
Copyright © 2000 International Society of Nephrology Terms and Conditions

4. Figure 2
(a) Double labeling for ED-1 [monocytes/macrophages (Mo/Mϕ), red] and proliferating cell nuclear antigen (PCNA; brown ×200). (b) ED-1 (upward arrow) and PCNA (downward arrow) ×1000. (c) Double labeling for α-smooth muscle actin (α-SMA) (myofibroblasts, red) and PCNA (brown; ×200). (d) α-SMA/PCNA–labeled cell (arrow; ×1000). (e) Collagen III staining 64 days postischemia (inner cortical region; ×200). (f) Collagen III staining in the 64-day nonischemic control (inner cortical region; ×200). (g) matrix metalloprotein-2 (MMP-2) glomerular staining eight-days postischemia (×400). (h) MMP-2 staining two-days postischemia (×200).
Kidney International  , DOI: ( /j x)
Copyright © 2000 International Society of Nephrology Terms and Conditions

5. Figure 3
Morphometric quantitation of PCNA positive cells. Symbols are: (▪) tubular; () interstitial; () other. *P < for tubule cells as compared with 0-day nonischemic controls. #P < for interstitial cells. “Other” cells included glomerular and vascular cells.
Kidney International  , DOI: ( /j x)
Copyright © 2000 International Society of Nephrology Terms and Conditions

6. Figure 3
Morphometric quantitation of PCNA positive cells. Symbols are: (▪) tubular; () interstitial; () other. *P < for tubule cells as compared with 0-day nonischemic controls. #P < for interstitial cells. “Other” cells included glomerular and vascular cells.
Kidney International  , DOI: ( /j x)
Copyright © 2000 International Society of Nephrology Terms and Conditions

7. Figure 3
Morphometric quantitation of PCNA positive cells. Symbols are: (▪) tubular; () interstitial; () other. *P < for tubule cells as compared with 0-day nonischemic controls. #P < for interstitial cells. “Other” cells included glomerular and vascular cells.
Kidney International  , DOI: ( /j x)
Copyright © 2000 International Society of Nephrology Terms and Conditions

8. Figure 4
Morphometric quantitation of TUNEL-positive cells. Symbols are: (▪) tubular; () interstitial; () other. *P < for tubule cells as compared with 0-day nonischemic controls. #P < for interstitial cells. “Other” cells included glomerular and vascular cells. Note that TUNEL-positive cells increased at day 2 and beyond had condensed chromatin and apoptotic bodies and other morphological evidence of apoptosis. TUNEL-positive tubule cells at one-day postischemia did not appear to have condensed chromatin, with homogeneously staining nuclei.
Kidney International  , DOI: ( /j x)
Copyright © 2000 International Society of Nephrology Terms and Conditions

9. Figure 4
Morphometric quantitation of TUNEL-positive cells. Symbols are: (▪) tubular; () interstitial; () other. *P < for tubule cells as compared with 0-day nonischemic controls. #P < for interstitial cells. “Other” cells included glomerular and vascular cells. Note that TUNEL-positive cells increased at day 2 and beyond had condensed chromatin and apoptotic bodies and other morphological evidence of apoptosis. TUNEL-positive tubule cells at one-day postischemia did not appear to have condensed chromatin, with homogeneously staining nuclei.
Kidney International  , DOI: ( /j x)
Copyright © 2000 International Society of Nephrology Terms and Conditions

10. Figure 4
Morphometric quantitation of TUNEL-positive cells. Symbols are: (▪) tubular; () interstitial; () other. *P < for tubule cells as compared with 0-day nonischemic controls. #P < for interstitial cells. “Other” cells included glomerular and vascular cells. Note that TUNEL-positive cells increased at day 2 and beyond had condensed chromatin and apoptotic bodies and other morphological evidence of apoptosis. TUNEL-positive tubule cells at one-day postischemia did not appear to have condensed chromatin, with homogeneously staining nuclei.
Kidney International  , DOI: ( /j x)
Copyright © 2000 International Society of Nephrology Terms and Conditions