Presentation is loading. Please wait.

Presentation is loading. Please wait.

Delayed Marrow Infusion in Mice Enhances Hematopoietic and Osteopoietic Engraftment by Facilitating Transient Expansion of the Osteoblastic Niche  Roberta.

Published byMargery McKenzie Modified over 5 years ago

Similar presentations

Presentation on theme: "Delayed Marrow Infusion in Mice Enhances Hematopoietic and Osteopoietic Engraftment by Facilitating Transient Expansion of the Osteoblastic Niche  Roberta."— Presentation transcript:

1 Delayed Marrow Infusion in Mice Enhances Hematopoietic and Osteopoietic Engraftment by Facilitating Transient Expansion of the Osteoblastic Niche  Roberta Marino, Satoru Otsuru, Ted J. Hofmann, Timothy S. Olson, Valeria Rasini, Elena Veronesi, Kelli Boyd, Mostafa Waleed Gaber, Caridad Martinez, Paolo Paolucci, Massimo Dominici, Edwin M. Horwitz  Biology of Blood and Marrow Transplantation  Volume 19, Issue 11, Pages (November 2013) DOI: /j.bbmt Copyright © Terms and Conditions

2 Figure 1 Kinetics of osteoblast proliferation and osteopoietic/hematopoietic chimerism. (A) Representative photomicrographs from 6 hours (left) and 48 hours (right) after TBI depicting the proliferation of the endosteal osteoblasts (arrows). (B) Time course of endosteal osteoblast proliferation in the metaphysis after TBI. Proliferation index as described in the text. Data points represent mean ± standard deviation. (C) Bone chimerism by time to cell infusion. Chimerism was defined as the fraction of GFP+ osteoblasts and osteocytes in the metaphysis and epiphysis, determined by immunohistochemical staining and microscopic evaluation. (D) Chimerism within the KLS subpopulation of marrow cells according to the intervals from TBI to cell infusion, as determined by multicolor flow cytometric analysis of marrow isolated from transplanted mice. (E) Bone chimerism, determined as in (C), after different marrow dosing schedules. Bars represent mean ± standard deviation values. Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt ) Copyright © Terms and Conditions

3 Figure 2 Comparison of osteopoietic and hematopoietic chimerism in irradiated versus shielded (nonirradiated) limbs according to time from TBI to marrow infusion. Total marrow cell, KLS marrow cell, and bone chimerism were determined in shielded versus irradiated legs 3 weeks post-transplantation, when donor cells were transplanted at (A) 6 hours or (B) 24 hours. Histograms are representative of the flow cytometric analyses for the bone marrow and KLS chimerism in shielded (- - -) and unshielded (—) legs. Total marrow cell and bone chimerism were determined in left and right legs (both unshielded during irradiation) of control mice 3 weeks post-transplantation, when donor cells were transplanted at (C) 6 hours or (D) 24 hours. In all graphs, bars represent the mean ± standard deviation (n = 10). NS, not statistically significant. Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt ) Copyright © Terms and Conditions

4 Figure 3 Independent analysis of irradiated and shielded (nonirradiated) limbs for osteopoietic and hematopoietic chimerism. Total marrow cell, KLS marrow cell, and bone chimerism were assessed in the irradiated (A) and shielded (nonirradiated) (B) legs of mice infused with donor cells 6 hours versus 24 hours after TBI. These data are derived from the same experiment as in Figure 2. Bars represent mean ± standard deviation values. NS, not statistically significant. Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt ) Copyright © Terms and Conditions

5 Figure 4 Engraftment of CD150+ CD48− CD41− (SLAM) cells in irradiated and shielded (nonirradiated) limbs. SLAM cells were measured in the bone marrow from the shielded or irradiated legs of mice transplanted at (A) 6 hours or (B) 24 hours after TBI and analyzed for chimerism and other engraftment parameters. The absolute SLAM count represents the number of SLAM cells per 106 viable marrow cells as determined by flow cytometry. Chimerism is the percentage of donor-derived (GFP+) SLAM cells. The absolute GFP+ SLAM count represents the number of GFP+ SLAM cells per 106 viable marrow cells. (C) Comparison of the absolute GFP+ SLAM count in the irradiated limb when marrow was infused 6 hours versus 24 hours after TBI. (D) Comparison of the absolute GFP+ SLAM count in the shielded (nonirradiated) limb when marrow was infused 6 hours versus 24 hours after TBI. In all panels, bars represent the mean ± standard deviation values. NS, not statistically significant. Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt ) Copyright © Terms and Conditions

6 Figure 5 Endosteal osteoblast proliferation in the irradiated versus shielded (nonirradiated) limbs. The photomicrographs depict H & E staining of femora taken from mice 24 hours after TBI. (A) Femur section from an irradiated limb; arrows indicate osteoblasts. (B) Femur section from shielded limb depicting regions of proliferating osteoblasts (multiple cell layers, left) and quiescent osteoblasts (single cell layer, right). (C) Femur section from a control (nonirradiated) mouse included for comparison; arrows indicate osteoblasts. (D) Selected region of a femur from a shielded limb demonstrating so-called plump osteoblasts in contrast to the more flattened cells seen elsewhere in the shielded limb (B) or during homeostasis (C). (E) Comparison of the magnitude of osteoblast proliferation, scored according to the proliferation index (see Methods) among femora from nonirradiated mice (controls), shielded limbs from irradiated mice, and irradiated limbs. Data were analyzed using a 1-way analysis of variance with Tukey's multiple comparison test comparing each experimental group with the controls. The bars represent mean ± standard deviation. Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt ) Copyright © Terms and Conditions

Download ppt "Delayed Marrow Infusion in Mice Enhances Hematopoietic and Osteopoietic Engraftment by Facilitating Transient Expansion of the Osteoblastic Niche  Roberta."

Similar presentations

Erik Ames, Salif Harouna, Colin Meyer, Lisbeth A. Welniak, William J

Erik Ames, Salif Harouna, Colin Meyer, Lisbeth A. Welniak, William J
Joseph H. Chewning, Weiwei Zhang, David A. Randolph, C

Joseph H. Chewning, Weiwei Zhang, David A. Randolph, C
Host-Derived CD8+ Dendritic Cells Protect Against Acute Graft-versus-Host Disease after Experimental Allogeneic Bone Marrow Transplantation  Michael Weber,

Host-Derived CD8+ Dendritic Cells Protect Against Acute Graft-versus-Host Disease after Experimental Allogeneic Bone Marrow Transplantation  Michael Weber,
High-Risk Acute Lymphoblastic Leukemia Cells with bcr-abl and Ink4a/Arf Mutations Retain Susceptibility to Alloreactive T Cells  Faith M. Young, Andrew.

High-Risk Acute Lymphoblastic Leukemia Cells with bcr-abl and Ink4a/Arf Mutations Retain Susceptibility to Alloreactive T Cells  Faith M. Young, Andrew.
Influence of Donor Microbiota on the Severity of Experimental Graft-versus-Host- Disease  Isao Tawara, Chen Liu, Hiroya Tamaki, Tomomi Toubai, Yaping Sun,

Influence of Donor Microbiota on the Severity of Experimental Graft-versus-Host- Disease  Isao Tawara, Chen Liu, Hiroya Tamaki, Tomomi Toubai, Yaping Sun,
Stromal-Derived Factor-1α and Interleukin-7 Treatment Improves Homeostatic Proliferation of Naïve CD4+ T Cells after Allogeneic Stem Cell Transplantation 

Stromal-Derived Factor-1α and Interleukin-7 Treatment Improves Homeostatic Proliferation of Naïve CD4+ T Cells after Allogeneic Stem Cell Transplantation 
IL-2–Targeted Therapy Ameliorates the Severity of Graft-versus-Host Disease: Ex Vivo Selective Depletion of Host-Reactive T Cells and In Vivo Therapy 

IL-2–Targeted Therapy Ameliorates the Severity of Graft-versus-Host Disease: Ex Vivo Selective Depletion of Host-Reactive T Cells and In Vivo Therapy 
Host conditioning with total lymphoid irradiation and antithymocyte globulin prevents graft-versus-host disease: the role of CD1-reactive natural killer.

Host conditioning with total lymphoid irradiation and antithymocyte globulin prevents graft-versus-host disease: the role of CD1-reactive natural killer.
Yumi Matsuzaki, Kentaro Kinjo, Richard C Mulligan, Hideyuki Okano 

Yumi Matsuzaki, Kentaro Kinjo, Richard C Mulligan, Hideyuki Okano 
Apoptotic Donor Leukocytes Limit Mixed-Chimerism Induced by CD40-CD154 Blockade in Allogeneic Bone Marrow Transplantation  Jian-ming Li, John Gorechlad,

Apoptotic Donor Leukocytes Limit Mixed-Chimerism Induced by CD40-CD154 Blockade in Allogeneic Bone Marrow Transplantation  Jian-ming Li, John Gorechlad,
Volume 25, Issue 9, Pages (September 2017)

Volume 25, Issue 9, Pages (September 2017)
LBH589 Enhances T Cell Activation In Vivo and Accelerates Graft-versus-Host Disease in Mice  Dapeng Wang, Cristina Iclozan, Chen Liu, Changqing Xia, Claudio.

LBH589 Enhances T Cell Activation In Vivo and Accelerates Graft-versus-Host Disease in Mice  Dapeng Wang, Cristina Iclozan, Chen Liu, Changqing Xia, Claudio.
Cytosine-Phosphorothionate-Guanine Oligodeoxynucleotides Exacerbates Hemophagocytosis by Inducing Tumor Necrosis Factor–Alpha Production in Mice after.

Cytosine-Phosphorothionate-Guanine Oligodeoxynucleotides Exacerbates Hemophagocytosis by Inducing Tumor Necrosis Factor–Alpha Production in Mice after.
Jacob Andrade, Shundi Ge, Goar Symbatyan, Michael S. Rosol, Arthur J

Jacob Andrade, Shundi Ge, Goar Symbatyan, Michael S. Rosol, Arthur J
Altered Homeostasis of CD4+ Memory T Cells in Allogeneic Hematopoietic Stem Cell Transplant Recipients: Chronic Graft-versus-Host Disease Enhances T Cell.

Altered Homeostasis of CD4+ Memory T Cells in Allogeneic Hematopoietic Stem Cell Transplant Recipients: Chronic Graft-versus-Host Disease Enhances T Cell.
Vascular Endothelial Cells Produce Soluble Factors That Mediate the Recovery of Human Hematopoietic Stem Cells after Radiation Injury  Garrett G. Muramoto,

Vascular Endothelial Cells Produce Soluble Factors That Mediate the Recovery of Human Hematopoietic Stem Cells after Radiation Injury  Garrett G. Muramoto,
A Role for TNF Receptor Type II in Leukocyte Infiltration into the Lung during Experimental Idiopathic Pneumonia Syndrome  Gerhard C. Hildebrandt, Krystyna.

A Role for TNF Receptor Type II in Leukocyte Infiltration into the Lung during Experimental Idiopathic Pneumonia Syndrome  Gerhard C. Hildebrandt, Krystyna.
Preactivation with IL-12, IL-15, and IL-18 Induces CD25 and a Functional High-Affinity IL-2 Receptor on Human Cytokine-Induced Memory-like Natural Killer.

Preactivation with IL-12, IL-15, and IL-18 Induces CD25 and a Functional High-Affinity IL-2 Receptor on Human Cytokine-Induced Memory-like Natural Killer.
Human Progenitor Cells Rapidly Mobilized by AMD3100 Repopulate NOD/SCID Mice with Increased Frequency in Comparison to Cells from the Same Donor Mobilized.

Human Progenitor Cells Rapidly Mobilized by AMD3100 Repopulate NOD/SCID Mice with Increased Frequency in Comparison to Cells from the Same Donor Mobilized.
Association of an Impaired Bone Marrow Microenvironment with Secondary Poor Graft Function after Allogeneic Hematopoietic Stem Cell Transplantation  Yuan.

Association of an Impaired Bone Marrow Microenvironment with Secondary Poor Graft Function after Allogeneic Hematopoietic Stem Cell Transplantation  Yuan.

Similar presentations

Ads by Google