Presentation is loading. Please wait.

Presentation is loading. Please wait.

Suction blistering the lesional skin of vitiligo patients reveals useful biomarkers of disease activity  James P. Strassner, BS, Mehdi Rashighi, MD, Maggi.

Published byClarence Watkins Modified over 5 years ago

Similar presentations

Presentation on theme: "Suction blistering the lesional skin of vitiligo patients reveals useful biomarkers of disease activity  James P. Strassner, BS, Mehdi Rashighi, MD, Maggi."— Presentation transcript:

1 Suction blistering the lesional skin of vitiligo patients reveals useful biomarkers of disease activity  James P. Strassner, BS, Mehdi Rashighi, MD, Maggi Ahmed Refat, MBBCh, MSc, Jillian M. Richmond, PhD, John E. Harris, MD, PhD  Journal of the American Academy of Dermatology  Volume 76, Issue 5, Pages e5 (May 2017) DOI: /j.jaad Copyright © 2016 American Academy of Dermatology, Inc. Terms and Conditions

2 Fig 1 Modified suction-blister technique. A, Graphic demonstrating T cells clustering near the epidermis, where the chemokine concentration is highest (top panel). After suction blistering, the epidermis forms the roof (blister), which becomes separated from the dermis (middle panel). T cells and chemokine at the site are present in the blister fluid and can be aspirated by fine needle aspiration (bottom panel). B, Representative photos from a patient with active vitiligo before blistering (left panel), the same site after blistering (middle panel), and of a nonlesional site (right panel). C, A study subject waits while the suction blisters form. Journal of the American Academy of Dermatology  , e5DOI: ( /j.jaad ) Copyright © 2016 American Academy of Dermatology, Inc. Terms and Conditions

3 Fig 2 CD8+ T-cell phenotyping and quantification. A, Representative flow plots of the T-cell infiltrate isolated from lesional, nonlesional, and healthy control skin. B, C-X-C motif chemokine receptor (CXCR) 3 expression on CD8+ T cells in lesional skin. C, Quantification of the CD8+ T-cell infiltrate of each sample normalized to 10,000 CD45+ cells for the defining group (left panel) and paired analysis of vitiligo subject samples (lesional vs nonlesional, right panel). E, Quantification of the CD8+ T-cell infiltrate of each sample normalized to 10,000 CD45+ cells for the validation group (left panel) and paired analysis of vitiligo subject samples (lesional vs nonlesional, right panel). D and F, Receiver operating characteristic curve for the defining group (D) and the validation group (F). Red dots mark threshold values described in the text. Journal of the American Academy of Dermatology  , e5DOI: ( /j.jaad ) Copyright © 2016 American Academy of Dermatology, Inc. Terms and Conditions

4 Fig 3 Chemokine protein levels in blister fluid. Quantification of C-X-C motif chemokine ligand (CXCL) 9 (A and C) or CXCL10 (B and D) in the blister fluid of the defining group (top panels) and validation group (bottom panels). Each panel is organized in the following manner: quantification of individual samples grouped by active lesional, active nonlesional, and healthy control (left), paired analysis of lesional and nonlesional blisters from vitiligo subjects (middle panel), and receiver operating characteristic curve (right). Red dots mark threshold values described in the text. Journal of the American Academy of Dermatology  , e5DOI: ( /j.jaad ) Copyright © 2016 American Academy of Dermatology, Inc. Terms and Conditions

5 Fig 4 Serum chemokine levels in patients with vitiligo and healthy controls. Serum levels of C-X-C motif chemokine ligand (CXCL) 9 and CXCL10 are reported for the defining group (A) and the validation group (B). C, Serum levels of CXCL9 (left) and CXCL10 (right) are compiled from both groups. D, Serum levels of CXCL11 (left) and IFN-γ (right). Journal of the American Academy of Dermatology  , e5DOI: ( /j.jaad ) Copyright © 2016 American Academy of Dermatology, Inc. Terms and Conditions

6 Fig 5 Concentrations of biomarkers CD8, CXCL9, and CXCL10 in a patient following conventional treatment. Subject began treatment at month 0, inconsistently followed treatment up to 4 months, and then consistently followed treatment from month 4 to month 8. A, T cells and chemokine protein reported at each visit (left panels). Representative slopes comparing lesional infiltrate to nonlesional infiltrate (right panels). B and C, CXCL9 concentration measured in the blister fluid (B) and serum (C) at each visit. D and E, CXCL10 concentration in the blister fluid (D) and serum (E) at each visit. Journal of the American Academy of Dermatology  , e5DOI: ( /j.jaad ) Copyright © 2016 American Academy of Dermatology, Inc. Terms and Conditions

7 Supplemental Fig 1 Gating strategy and flow controls. A, CD8+ and CD4+ cells were gated in the following manner: singlets, cell gate, live cells by dead stain exclusion, all CD45+ cells, and all CD3+ T cells. B, Stained whole blood was used to make the fluorescence minus one (FMO) controls to draw the C-X-C motif chemokine receptor (CXCR) 3 gate. Journal of the American Academy of Dermatology  , e5DOI: ( /j.jaad ) Copyright © 2016 American Academy of Dermatology, Inc. Terms and Conditions

8 Supplemental Fig 2 CD3 and CD4 quantification. A-C, Quantification of CD3+ and CD4+ cells normalized to CD45+ cells in the skin of vitiligo subjects and healthy controls. Results are shown separately for the defining group (A), validation group (B), and both data sets combined (C). D, Receiver operating characteristic analysis of compiled CD3 counts from both groups. Red dot signifies the normalized CD3 T-cell threshold value 6927, which achieves a sensitivity of 81.4% and specificity of 75.5%. Journal of the American Academy of Dermatology  , e5DOI: ( /j.jaad ) Copyright © 2016 American Academy of Dermatology, Inc. Terms and Conditions

9 Supplemental Fig 3 Skin C-X-C motif chemokine ligand (CXCL) 11 and interferon-γ (IFN-γ) protein concentration. Quantification of CXCL11 (A) and IFN-γ (B) protein concentration by enzyme-linked immunosorbent assay of blister fluid in vitiligo subjects and healthy controls. Journal of the American Academy of Dermatology  , e5DOI: ( /j.jaad ) Copyright © 2016 American Academy of Dermatology, Inc. Terms and Conditions

Download ppt "Suction blistering the lesional skin of vitiligo patients reveals useful biomarkers of disease activity  James P. Strassner, BS, Mehdi Rashighi, MD, Maggi."

Similar presentations

Flow cytometry imaging identifies rare TH2 cells expressing thymic stromal lymphopoietin receptor in a “proallergic” milieu  Amanda J. Reefer, MS, Kathryn.

Flow cytometry imaging identifies rare TH2 cells expressing thymic stromal lymphopoietin receptor in a “proallergic” milieu  Amanda J. Reefer, MS, Kathryn.
A girl with a solitary red bulla

A girl with a solitary red bulla
Striking leflunomide efficacy against refractory cutaneous sarcoidosis

Striking leflunomide efficacy against refractory cutaneous sarcoidosis
Monocyte chemoattractant chemokines in cystic fibrosis

Monocyte chemoattractant chemokines in cystic fibrosis
Yul W. Yang, MD, PhD, David J. DiCaudo, MD 

Yul W. Yang, MD, PhD, David J. DiCaudo, MD 
Reduced TH1/TH17 CD4 T-cell numbers are associated with impaired purified protein derivative–specific cytokine responses in patients with HIV-1 infection 

Reduced TH1/TH17 CD4 T-cell numbers are associated with impaired purified protein derivative–specific cytokine responses in patients with HIV-1 infection 
Allison Hanlon, MD, PhD, June Kim, MD, David J. Leffell, MD 

Allison Hanlon, MD, PhD, June Kim, MD, David J. Leffell, MD 
Diffuse hyperpigmented plaques as cutaneous manifestation of multicentric Castleman disease and treatment with thalidomide: Report of three cases  Xiaoqing.

Diffuse hyperpigmented plaques as cutaneous manifestation of multicentric Castleman disease and treatment with thalidomide: Report of three cases  Xiaoqing.
Image registration of sequential transparent photographs to localize and detect new versus recurrent tumors in dermatologic and Mohs micrographic surgery 

Image registration of sequential transparent photographs to localize and detect new versus recurrent tumors in dermatologic and Mohs micrographic surgery 
Baldness reversed by chemotherapy

Baldness reversed by chemotherapy
Expression of PD-L1 in mastocytosis

Expression of PD-L1 in mastocytosis
Idiopathic eruptive macular pigmentation associated with pregnancy and Hashimoto thyroiditis  Danica Milobratovic, MD, Sanja Djordjevic, MD, Jelica Vukicevic,

Idiopathic eruptive macular pigmentation associated with pregnancy and Hashimoto thyroiditis  Danica Milobratovic, MD, Sanja Djordjevic, MD, Jelica Vukicevic,
Elana Putterman, BS, Leslie Castelo-Soccio, MD, PhD 

Elana Putterman, BS, Leslie Castelo-Soccio, MD, PhD 
Deficiency of serum concentration of 25-hydroxyvitamin D correlates with severity of disease in chronic plaque psoriasis  Federica Ricceri, MD, Leonardo.

Deficiency of serum concentration of 25-hydroxyvitamin D correlates with severity of disease in chronic plaque psoriasis  Federica Ricceri, MD, Leonardo.
Photo-induced Stevens-Johnson syndrome

Photo-induced Stevens-Johnson syndrome
Increased blood levels of NKG2D+CD4+ T cells in patients with alopecia areata  Yong Hyun Jang, MD, PhD, Jin Kyeong Choi, PhD, Yun Hwan Jang, MD, Sun Young.

Increased blood levels of NKG2D+CD4+ T cells in patients with alopecia areata  Yong Hyun Jang, MD, PhD, Jin Kyeong Choi, PhD, Yun Hwan Jang, MD, Sun Young.
A double-blind, placebo-controlled, phase-II clinical trial to evaluate oral simvastatin as a treatment for vitiligo  Stefan G. Vanderweil, MD, Shinya.

A double-blind, placebo-controlled, phase-II clinical trial to evaluate oral simvastatin as a treatment for vitiligo  Stefan G. Vanderweil, MD, Shinya.
Expression of programmed death receptor ligand 1 in melanoma may indicate tumor progression and poor patient survival  Junna Oba, MD, PhD, Takeshi Nakahara,

Expression of programmed death receptor ligand 1 in melanoma may indicate tumor progression and poor patient survival  Junna Oba, MD, PhD, Takeshi Nakahara,
Atypical lymphocytic reaction with epidermotropism and lymphocytic vasculopathic reaction (lymphocytic vasculitis) after treatment with imiquimod  Jesús.

Atypical lymphocytic reaction with epidermotropism and lymphocytic vasculopathic reaction (lymphocytic vasculitis) after treatment with imiquimod  Jesús.
Re-emergence of anti-topoisomerase I antibody with exacerbated development of skin sclerosis in a patient with systemic sclerosis  Yasuhito Hamaguchi,

Re-emergence of anti-topoisomerase I antibody with exacerbated development of skin sclerosis in a patient with systemic sclerosis  Yasuhito Hamaguchi,

Similar presentations

Ads by Google