1. Corticotropin-releasing hormone inhibits in vitro oocyte maturation in mice 
Erasmia Kiapekou, M.D., Evangelia Zapanti, M.D., Dionysia Voukelatou, B.Sc., Theodora Mavreli, B.Sc., Konstantinos Stefanidis, M.D., Peter Drakakis, M.D., George Mastorakos, M.D., Dimitris Loutradis, M.D. 
Fertility and Sterility 
Volume 95, Issue 4, Pages e1 (March 2011)
DOI: /j.fertnstert
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Nuclear maturation rate of mouse oocytes from preantral follicles cultured without (control) and with different CRH (10−11, 10−10, 10−9, 10−7, and 10−6 mol/L) and antalarmin (10−8 and 10−6 mol/L) concentrations. The maturation rate for each stage of maturation is expressed as the percentage of the total number of GV, or GVBD, or PB stage oocytes over the total number of COCs in the same culture. ∗Statistically significant difference from the corresponding control group (P<.05). #Statistically significant difference from the cultures with addition of the same concentration of CRH alone (P<.05).
Fertility and Sterility  , e1DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

3. Gel electrophoresis of RT-PCR products for CRH-R1 and GAPDH control in mouse preantral follicles, COCs, GV stage oocytes, and PB oocytes with (+) and without (−) reverse transcriptase. (A) CRH-R1, fragment 230, (B) GAPDH control; preantral follicles (PR) (+, −), COCs (+, −), GV oocytes (GV) (+, −), and 1st PB oocytes (PB) (+, −). The detection of GAPDH mRNA in mouse preantral follicles, COCs, GV stage oocytes, and PB oocytes confirmed the integrity of the RNA isolation and the RT-PCR process. (C) Gel electrophoresis of RT-PCR products for CRH-R1 in follicles from culture days 4 and 8, cultured without (control) and with different CRH (10−9, 10−7, and 10−6 mol/L) and antalarmin (10−8 and 10−6 mol/L) concentrations. (D) Gel electrophoresis of RT-PCR products for CRH-R1 in follicles from culture day 11, cultured without (control) and with different CRH (10−9, 10−7, and 10−6 mol/L) and antalarmin (10−8 and 10−6 mol/L) concentrations.
Fertility and Sterility  , e1DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions