1. By: Zatilfarihiah Rasdi
Biochemistry II
BCM311

2. Objectives Upon completion of this course, students should be able to:
Define the basic terms and concepts in enzyme kinetics, metabolism and electron transport system
Describe the functions, reactions and control of selected meatbolic pathways of carbohydrates, amino acids, lipids metabolisms
Show the production and consumption of “high energy” compounds like ATP, production and consumption of reducing power in the pathways studied

3. Lesson plan Week Contents 1 Introduction to biochemistry 2
Bioenergetics 3 4
TEST 1 5
Enzyme kinetics 6
Carbohydrate metabolism 7
TEST 2 8
Fat metabolism 9
Amino acids catabolism 10
TEST 3 11
Term-break 12
Metabolic interrelationship 13
TEST 4 14
Revision 15

4. Lab class Reference Tests - 40% Final exam. - 60%
Campbell, M.K., and Farrell, S.O. (2009) Biochemistry, 7th ed.
Thompson Brooks/Cole
Boyer, R. (2005)
Concepts in Biochemistry, 3rd ed.
Wiley
Additional text
Voet, D.J, Voet, G.V, and Pratt, C.W (2008)
Principle of Biochemistry, 3rd ed.
Colorimetric tests for carbohydrates
Chromatography
Saponification value
Acid value
Qualitative tests for amino acid
Tests for proteins
The pH meter
Paper chromatography
Assay for proteins, reducing sugar
Determination of KM and Vmax
Electrophoresis and chromatography
practice in BCM312
Assessment
Tests - 40%
Final exam. - 60%