1. Development of an NPM1/MLF1 D-FISH Probe Set for the Detection of t(3;5)(q25;q35) Identified in Patients with Acute Myeloid Leukemia 
Umut Aypar, Ryan A. Knudson, Kathryn E. Pearce, Anne E. Wiktor, Rhett P. Ketterling 
The Journal of Molecular Diagnostics 
Volume 16, Issue 5, Pages (September 2014)
DOI: /j.jmoldx
Copyright © 2014 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

2. Figure 1
A: Probe schematic for the four BACs labeled in red, CTD-2332C24, RP11-615G7, RP11-143J5, and CTD-3046G9, spanning the 3q25 MLF1 gene region. B: Probe schematic for the three BACs labeled in green, RP I20, RP11-117L6, and CTD-2366L8, spanning the 5q35 NPM1 gene region.
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2014 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

3. Figure 2
A: Representative interphase FISH signal patterns that may occur for the NPM1/MLF1 probe set. Normal nuclei with a 2R2G signal pattern. B: Normal nuclei with coincidental overlap of R and G signals resulting in a 1R1G1F signal pattern or abnormal nuclei due to loss of the second fusion signal. C: Abnormal nuclei with a typical D-FISH signal pattern of 1R1G2F. D–F: Abnormal nuclei with atypical signal patterns of 2R1G (D), 3R2G (E), and 3R1G (F).
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2014 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions