1. Different Genes ~ Protein Primary Structure
Different Primary Sequences ~ 3D Structure
Genes << Primary Structure << 3D Structure
Relative scale of conservation

2. Gene Protein Bioinformatics - Mechanism of action / biological role
- Evolutionary studies, protein folding...
- Therapeutic applications: drug/vaccine design
- Biotechnological applications: diagnostics, products

3. 1) Domain Organisation and Function
Collagen Integrin
FN FN FN12-14 I II I
III
III
III
III
III
III
III
III
III I
S, S
Fibrin Integrin Heparin Fibrin
Heparin Integrin
- involved in cell adhesion, migration, matrix formation
- binds to fibrin, integrin, heparin, collagens, gelatin, DNA
- modular architecture consisting of type I, II and III repeats
- FN7-10 binds integrins via the amino acid sequence RGD
- FN12-14 binds heparin and integrin

4. Heparin
binding
Integrin
binding

5. Integrin
binding
Heparin
binding

6. GLOBAL INFORMATION FROM GENOMES
2D GE
BIO-INFO
MASS
SPEC

7. PROTEOMICS - GLOBAL INFORMATION FROM GENOMES (mRNA EXPRESSION STUDIES)
- CLASSICAL REDUCTIONIST APPROACHES
PROTEOMICS

8. Expression Strategy Purification Strategy
BIOCHEMICAL REDUCTIONIST APPROACHES
# aa, mol. wt.,
pI, extinction coeff.
Glycosylation
Signal sequence
Transmembrane segments
Modifications (> 200)
Protease cleavage sites
Secondary structure prediction
Function
Fold, family, homologues
3D structure
Mutagenesis data
Higher order complexes
Expression
Strategy
Purification
Strategy

16. P16893 N C
SS A-domain TRM RGD Proline-rich TM/CYT
200 aa region