1. Group Name
MRSA1
Group member name
Raluca Datcu
Mihaela Oprea
Oana Barbu
Anita Forslund
Cathrine Fladeby
Mona Holberg

2. Isolates Sample name Subtype Notes ERR070039-P11 ST2371
From Patient 11
ERR P15
From Patient 15
ERR P26
From Patient 26
ERR HW
From Health Care Worker - misses the ermC plasmid
ERR not-outbreak
ST1
Not part of the outbreak

3. Result of species identification (KmerFinder)
Strain
Species acc. to Harris et al 2013
Predicted species
Closest matching strain
Template coverage
ERR P11
S. aureus
S. aureus subsp. aureus HO
97.46
ERR P15
ERR P26
97.44
ERR HW
ERR not-outbreak
MSSA 476
98.59

4. Representative KmerFinder result

5. Result of Multilocus Sequence Typing (MLST)
Strain
ST acc. to Harris et al 2014
Predicted ST
ERR P11
ST-2371
ERR P15
ERR P26
ERR HW
ERR not-outbreak
ST-1

6. Representative Multilocus Sequence Typing result

7. Result of antibiotics resistance analysis
Isolate
Cefoxitin/Methicillin (MecA)
Cipro-floxacin (chromo-somal mutation)
Erythro-mycin (Erm(A), Erm(B), Erm(C))
Fusidic acid (chromo-somal mutation)
Gentamicin (aac(6’)-aph(2''))
Mupirocin (chromo-somal mutation)
Rifampicin (chromo-somal mutation)
Tetra-cycline (tetC, tetM, tetR, tetS
Vancomycin (Chromo-somal mutations or the vanA or vanB gene cluster.)
Neomycin (aadD, aac(6')-aph(2''))
P11
Res
Unknown
Sus
P15
P26 HW
non-out-break
How is the agreement with the results presented in Harris et al 2013?
Good agreement, but for the HW isolate our DNA did not contained erm genes,
and Harris identified both susceptible and resistant colonies to erythromycin

8. Result of plasmid analysis
Isolate
rep10
repUS12
rep20
rep5
rep16
rep7
ERR P11
NODE 1
NODE 25
NODE 37 37 -
ERR P15
NODE 15
ERR P26
NODE 9
NODE 5
NODE 80
ERR HW
NODE 142
ERR not-outbreak
NODE 2
NODE 8
For each strain write which node the replicon was found on. If not found, leave the cell empty.
Which plasmid is the ermC gene on?
- PLASMID rep10

9. Distance matrix made by NDtree and tree made with neighbour-joining

10. Tree made with neighbour-joining

11. Distance matrix made by CSIPhylogeny and tree made with neighbour-joining

12. Tree made with neighbour-joining