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Fig. 4. In vivo analysis of slpA mutant in the Syrian Golden hamster.

Published byWidya Agusalim Modified over 5 years ago

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Presentation on theme: "Fig. 4. In vivo analysis of slpA mutant in the Syrian Golden hamster."— Presentation transcript:

1 Fig. 4. In vivo analysis of slpA mutant in the Syrian Golden hamster.
In vivo analysis of slpA mutant in the Syrian Golden hamster. (A) Times to experimental end point of animals infected with R20291 (black line), FM2.5 (dark blue line), and FM2.5RW (light blue line), respectively. Each line represents six animals. (B) Total CFUs and spore CFUs (after heat treatment at 56°C for 20 min) were determined for lumen-associated (LA) and tissue-associated (TA) bacteria recovered from cecum (CE) and colon (COL) of infected animals and quantified at experimental end point (R20291 and FM2.5RW) or at 14 days after infection (FM2.5). Means and SEs are shown. The horizontal dashed line indicates the limit of detection. None of the observed differences are statistically significant. (C and D) Relative toxin activity of filtered gut samples on HT-29 (toxin A) and Vero cells (toxin B), respectively. Values represent the reciprocal of the first dilution in which cell morphology was indistinguishable from untreated wells. Samples were taken at experimental end point (R20291 and FM2.5RW) or at 14 days after infection (FM2.5). *P < 0.05 and **P < 0.01, determined using a two-tailed nonparametric Mann-Whitney test; NS, not significant. Joseph A. Kirk et al., Sci Transl Med 2017;9:eaah6813 Published by AAAS

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