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A pilot study of a triple antimicrobial-bonded Dacron graft for the prevention of aortic graft infection  Ibrahim Aboshady, MD, Issam Raad, MD, Aamir.

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Presentation on theme: "A pilot study of a triple antimicrobial-bonded Dacron graft for the prevention of aortic graft infection  Ibrahim Aboshady, MD, Issam Raad, MD, Aamir."— Presentation transcript:

1 A pilot study of a triple antimicrobial-bonded Dacron graft for the prevention of aortic graft infection  Ibrahim Aboshady, MD, Issam Raad, MD, Aamir S. Shah, MD, Deborah Vela, MD, Tanya Dvorak, PhD, Hazim J. Safi, MD, L. Maximilian Buja, MD, Kamal G. Khalil, MD  Journal of Vascular Surgery  Volume 56, Issue 3, Pages (September 2012) DOI: /j.jvs Copyright © 2012 Society for Vascular Surgery Terms and Conditions

2 Fig 1 The dendrogram and virtual banding patterns after repetitive sequence-based polymerase chain reaction (rep-PCR) showed that the inoculated Staphylococcus aureus (SA) strain (ATCC 29213) recovered from the specimens was the same as the strain in the initial inoculum. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2012 Society for Vascular Surgery Terms and Conditions

3 Fig 2 Representative images of aortic specimens and adjacent graft material stained with (I) hematoxylin and eosin, (II) Movat pentachrome, and Gram stain. A and B, Images from pig 2 (group A) show a small cluster of gram-positive cocci and mild neutrophilic infiltration near the graft implantation site. C and D, Images from pig 4 (group B) show multiple clusters of gram-positive cocci (arrows) and abscess formation (asterisk). E and F, Images from pig 6 (group C) show negative Gram staining. Bar = 200 μm in B and F and 1 mm in D. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2012 Society for Vascular Surgery Terms and Conditions

4 Fig 3 High-power magnification image (bar = 20 μm) shows dense clusters of gram-positive cocci in the perigraft tissue of pig 4 (group B). Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2012 Society for Vascular Surgery Terms and Conditions

5 Fig 4 Images of abscess formation in specimens from group B pigs. Low-power magnification of aortic specimens are shown from (A) pig 3 and (B) pig 4. Corresponding high-power magnification images of aortic specimens are shown from (C) pig 3 and (D) pig 4. C, Neutrophilic infiltration is seen surrounding a core of graft and gram-positive bacterial remnants (br). D, An accumulation of mostly dead neutrophils, fibrin, and loose connective tissue is shown. A large cluster of gram-positive cocci is seen in the upper left corner (bc). Bar = 2 mm in A and 100 μm in C. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2012 Society for Vascular Surgery Terms and Conditions

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