1. Volume 140, Issue 7, Pages 2000-2008.e2 (June 2011)
Loss of Adiponectin Promotes Intestinal Carcinogenesis in Min and Wild-type Mice 
Michihiro Mutoh, Naoya Teraoka, Shinji Takasu, Mami Takahashi, Kunishige Onuma, Masafumi Yamamoto, Naoto Kubota, Takamoto Iseki, Takashi Kadowaki, Takashi Sugimura, Keiji Wakabayashi 
Gastroenterology 
Volume 140, Issue 7, Pages e2 (June 2011)
DOI: /j.gastro
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2. Figure 1
Serum adiponectin (APN) levels and expression levels of APN receptors in APN-deficient Min mice. (A) Serum APN levels for 12-week-old male APN+/+Min, APN+/−Min, and APN−/−Min mice (n = 3) and for 12-week-old male APN+/+ mice (n = 4) were evaluated by enzyme-linked immunosorbent assay. (B) Liver samples from 12-week-old male APN-deficient Min mice (n = 3) and for 12-week-old male APN+/+ C57BL/6J mice (n = 3) were analyzed for APN receptors, Adipo-R1 and -R2 by reverse transcription polymerase chain reaction analysis. Glyceraldehyde-3-phosphate dehydrogenase was employed as an internal control. WT, wild-type mice.
Gastroenterology  , e2DOI: ( /j.gastro )
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3. Figure 2
Phosphorylated−AMP-activated protein kinase (p-AMPK) and -Akt levels in adiponectin (APN)-deficient mice and its derived fibroblasts. Relative p-AMPK levels in the small intestine (A) and colon (B) of 9-week-old male APN+/+, APN+/−, and APN−/− C57BL/6J mice without azoxymethane treatment (n = 4 each), respectively, were evaluated by enzyme-linked immunosorbent assay (ELISA). (C) Relative p-AMPK levels in the liver of 12-week-old male APN+/+Min, APN+/−Min, and APN−/−Min mice (n = 4 each) were evaluated by ELISA. (D) Relative p-Akt/total Akt levels in the small intestine of AOM-treated 55-week-old male APN+/+, APN+/−, and APN−/− C57BL/6J mice (n = 6 each) were evaluated by ELISA. Black, APN+/+; gray, APN+/−; white, APN−/−. Data are mean ± standard error. **P < .01. *P < .05. (E) Effects of insulin (1 μg/mL for 5 and 10 minutes treatment) and interleukin-6 (IL-6; 50 ng/mL for 5 and 10 minutes) on the protein expression of p-Akt and -Erk, and Bcl-2 in fibroblasts derived from APN+/+ and APN−/− C57BL/6J mice are shown. β-actin was a loading control. Density of the bands was evaluated by NIH image, and the relative level was further normalized by β-actin.
Gastroenterology  , e2DOI: ( /j.gastro )
Copyright © 2011 AGA Institute Terms and Conditions