Download presentation
Presentation is loading. Please wait.
Published byBùi Ngô Modified over 5 years ago
1
A Convenient Nucleic Acid Test on the Basis of the Capillary Convective PCR for the On-Site Detection of Enterovirus 71 Shiyin Zhang, Yanyan Lin, Jin Wang, Penglin Wang, Jieyu Chen, Miaoge Xue, Shuizhen He, Wenbin Zhou, Feihai Xu, Pingguo Liu, Pinghei Chen, Shengxiang Ge, Ningshao Xia The Journal of Molecular Diagnostics Volume 16, Issue 4, Pages (July 2014) DOI: /j.jmoldx Copyright © 2014 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions
2
Figure 1 Schematic view of the D-tube.
The Journal of Molecular Diagnostics , DOI: ( /j.jmoldx ) Copyright © 2014 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions
3
Figure 2 Comparisons between the CCPCR instrument and a conventional RNA reverse transcription thermocycler. Lanes 1 to 6, RNA transcript using the CCPCR instrument; lanes 7 to 12, RNA transcript by traditional thermocycler. Lanes 1 and 7, 100 TCID50/mL; lanes 2 and 8, 10 TCID50/mL; lanes 3 and 9, 1 TCID50/mL; lanes 4 and 10, 0.1 TCID50/mL; lanes 5 and 11, 0.01 TCID50/mL; lanes 6 and 12, negative. M, DNA marker. The Journal of Molecular Diagnostics , DOI: ( /j.jmoldx ) Copyright © 2014 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions
4
Figure 3 A: The steps used for amplification and detection by ciNAT. B: Schematic diagram of test result readout: invalid detection (tube 1), negative detection (tube 2), and positive detection (tube 3). C: Results of serially diluted nucleic acid detected by ciNAT. Tubes 1 to 5, diluted nucleic acid in 10-fold from 100 to 0.01 TCID50/mL; tube 6, negative control. The Journal of Molecular Diagnostics , DOI: ( /j.jmoldx ) Copyright © 2014 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions
Similar presentations
© 2024 SlidePlayer.com. Inc.
All rights reserved.