1. Rom2 protein levels in the ccr4Δ, khd1Δ ccr4Δ, ccr4Δ mpk1Δ, and khd1Δ ccr4Δ mpk1Δ mutant strains.
Rom2 protein levels in the ccr4Δ, khd1Δ ccr4Δ, ccr4Δ mpk1Δ, and khd1Δ ccr4Δ mpk1Δ mutant strains. The Rom2myc protein levels in wild-type, ccr4Δ, khd1Δ ccr4Δ, ccr4Δmpk1Δ, and ccr4Δ khd1Δ mpk1Δ cells are shown. Wild-type (180-m-1D4A), khd1Δ ccr4Δ (180-m-3D), khd1Δ ccr4Δ mpk1Δ (180-m-7A), ccr4Δ (180-m-6B), and ccr4Δ mpk1Δ (180-m-4C) cells harboring the ROM2myc construct were cultured to mid-logarithmic phase in YPD containing 10% sorbitol medium and collected, and total protein was prepared. The Rom2myc proteins were quantified by Western blotting as described in Materials and Methods. Pgk1 protein was included as a quantity control. The protein levels are indicated as percentages of wild-type levels and represent the means ± standard deviations from three independent experiments.
Xia Li et al. mSphere 2016; doi: /mSphere