1. A Novel Mouse Gene, Sh3yl1, is Expressed in the Anagen Hair Follicle
Noriaki Aoki, Kaoru Ito, Masaaki Ito 
Journal of Investigative Dermatology 
Volume 114, Issue 5, Pages (May 2000)
DOI: /j x
Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

2. Figure 1
The predicted protein sequence of Sh3yl1. Sequences in the carboxyl terminus corresponding to the SH3 domain are boxed. The nucleotide sequence was registered in DDBJ, EMBL, and GenBank databases, and its accession number is D85926.
Journal of Investigative Dermatology  , DOI: ( /j x)
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3. Figure 2
Predicted amino acid sequences of Sh3yl1, YHRO 16c, Ysc 84, and YFRO 24c. Identical residues are shaded. The carboxyl-terminal SH3 domain is boxed. Dashed lines are gaps introduced to maximize identity.
Journal of Investigative Dermatology  , DOI: ( /j x)
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4. Figure 3
Domain diagram of Sh3yl1, YHRO 16c, and YFRO 24c shows the homology among the three proteins. The boundaries of the domain are delineated by amino acid numbers. The percentage identity for YHRO 16c between each domain is given. Amino-terminal domains (hatched areas) and carboxyl-terminal domains (black areas) are the regions most highly conserved among these proteins. In addition, another homologous region following the amino-terminal domain is observed between YHRO 16c and YFRO 24c (cross-hatched areas).
Journal of Investigative Dermatology  , DOI: ( /j x)
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5. Figure 4
Amino-acid alignment of SH3 domains of Sh3yl1 and representative SH3-domain-containing proteins. The amino acid of the first residue of each sequence is given in parentheses. Residues that are identical are shaded. Dashes denote gaps in the sequence to maximize alignment.
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6. Figure 5
Sh3yl1 transcript can be detected in various mouse tissues. Total RNA (10 μg) from the indicated mouse tissues was electrophoresed, transferred to nitrocellulose filter, and hybridized to 32P-labeled full-length Sh3yl1 cDNA probe. The blot was subsequently rehybridized with the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) probe. The RNA markers are indicated at the right.
Journal of Investigative Dermatology  , DOI: ( /j x)
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7. Figure 6
Sh3yl1 transcript is expressed at a higher level in anagen phase during two hair cycles. Total RNA (10 μg) prepared from mouse dorsal skin ranging from day 1 to day 42 was hybridized with Sh3yl1 cDNA probes. The blot was subsequently rehybridized with the 32P-end-labeled oligonucleotide derived from 18S rRNA probe.
Journal of Investigative Dermatology  , DOI: ( /j x)
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8. Figure 7
Quantitation of mRNA levels of Sh3yl1. The northern blots were scanned with BioImaging Analyzer BAS 2000 and the resulting values were normalized to the relative level of 18S rRNA, which was normalized to the maximum level of the transcript observed at day 9.
Journal of Investigative Dermatology  , DOI: ( /j x)
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9. Figure 8
Sh3yl1 transcript can be detected in anagen hair follicle. In situ hybridization was performed in mouse skin at day 36 corresponding to anagen VI. Bright field (a, c, e) and dark field (b, d, f) views of longitudinal sections. (e, f) High magnifications of (a) and (b). Note that Sh3yl1 transcripts were detected in the hair bulb including the matrix area (m), and hair shaft, IRS, and ORS in the lower half of the follicle in anagen VI, whereas the occasionally strong signal in hair shaft was due to light diffraction by highly keratinized material (b). Sense probe produced a random signal (c, d). Scale bars: (a, c) 100 μm; (e) 50 μm.
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10. Figure 9
Sh3yl1 protein can be detected in mouse kidney. Extracts of kidney (lane 1) and telogen (lane 2) and anagen (lane 3) skins were subjected to Western blotting with affinity purified anti-Sh3yl1 antibody. Sites of molecular weight markers are noted on the left.
Journal of Investigative Dermatology  , DOI: ( /j x)
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