1. Inhaled carbon monoxide inhibits intimal hyperplasia and provides added benefit with nitric oxide 
Kathleen G. Raman, MD, Joel E. Barbato, MD, Emeka Ifedigbo, BS, Brett A. Ozanich, BS, Mazen S. Zenati, MD, PhD, Leo E. Otterbein, PhD, Edith Tzeng, MD 
Journal of Vascular Surgery 
Volume 44, Issue 1, Pages (July 2006)
DOI: /j.jvs
Copyright © 2006 The Society for Vascular Surgery Terms and Conditions

2. Fig 1
Effect of inhaled carbon monoxide (CO) on the development of intimal hyperplasia after balloon angioplasty. A, Quantification of intimal and medial areas 3 weeks after balloon injury in pigs treated with 60 minutes of inhaled CO (250 ppm) or room air before surgery. B, Carboxyhemoglobin (Carboxy Hb) levels measured in pigs treated with air, 60 minutes of CO pretreatment, or 60 minutes of CO pretreatment followed by intraoperative CO administration (n = 5 for all time points except 120 minutes where n = 4; arrow indicates time of injury). C, Quantification of intimal and medial areas 3 weeks after balloon injury in pigs treated with 60 minutes of CO pretreatment and intraoperative CO administration versus oxygen. *P < .001 vs air treated controls. Data are reported ± SEM.
Journal of Vascular Surgery  , DOI: ( /j.jvs )
Copyright © 2006 The Society for Vascular Surgery Terms and Conditions

3. Fig 2
Representative cross-sections of pig iliac arteries 3 weeks after angioplasty. Sections were stained with hematoxylin and eosin (A, C, E) or viewed under autofluorescence (B, D, F) at ×4 magnification. A-B, Uninjured pig iliac artery. C-D, Balloon-injured pig iliac artery from a pig treated with oxygen. E-F, Balloon-injured pig iliac artery from a pig treated with 60 minutes of inhaled CO before surgery and intraoperative CO. Arrows indicate the internal elastic lamina.
Journal of Vascular Surgery  , DOI: ( /j.jvs )
Copyright © 2006 The Society for Vascular Surgery Terms and Conditions

4. Fig 3
Representative cross-sections of pig iliac arteries 3 weeks after angioplasty in combined carbon monoxide (CO) and gene transfer treatment. Sections were stained with hematoxylin and eosin (A, C, E, G) or viewed under autofluorescence (B, D, F, H) at ×4 magnification. A-B, Pig iliac artery treated with AdlacZ and oxygen. C-D, AdlacZ and CO treated. E-F, AdiNOS and oxygen treated. G-H, AdiNOS and CO treated. Arrows indicate the internal elastic lamina.
Journal of Vascular Surgery  , DOI: ( /j.jvs )
Copyright © 2006 The Society for Vascular Surgery Terms and Conditions

5. Fig 4
Quantification of intimal and medial areas 3 weeks after balloon injury in pigs treated with AdlacZ or AdiNOS along with preoperative and intraoperative carbon monoxide (CO) administration or air. *P < .001 ves AdlacZ + air; †P > .01 vs AdlacZ + CO and AdiNOS + air. Data are reported ± SEM.
Journal of Vascular Surgery  , DOI: ( /j.jvs )
Copyright © 2006 The Society for Vascular Surgery Terms and Conditions