1. Figure e-1 A B C
Wild-type TDP-43
G3BP
Merge+DAPI
Figure e-1. A TDP-43 fragment and mutant FUS form stress granules.
Cells expressing wild-type TDP-43 (A-C), p35f (D-F), wild-type FUS (G-I), or mutant P525L FUS (J-K) tagged with V5 at the C-terminus and GFP-G3BP (green), were labeled with anti-V5 antibody (red). Cytoplasmic IBs formed in cells expressing p35f, and mutant P525L FUS colocalized with the stress granule marker, G3BP. Scale bar = 10 μm. D E F
p35f
G3BP
Merge+DAPI G H I
Wild-type FUS
G3BP
Merge+DAPI J K L
P525L FUS
G3BP
Merge+DAPI