1. Sequencing at 10,000x using Illumina paired reads
9 mut. alleles in 10,000
3 mut. alleles in 10,000
Without error correction : 65 mut. alleles in 10,000
S. Marsilio et al. Supplementary Figure 3A

2. Sequencing at 100,000x doubles the sensitivity
4 mut. alleles in 10,000
1-2 mut. alleles in 10,000
S. Marsilio et al. Supplementary Figure 3B

3. 1,000,000x does not significantly improve sensitivity
3 mut. alleles in 10,000
1 mut. alleles in 10,000
S. Marsilio et al. Supplementary Figure 3C

4. Sensitivity of detecting transitions versus transversions
>5 transition mutant alleles in 10,000
4 mutant alleles in 10,000
>1 transversion mutant alleles in 10,000
S. Marsilio et al. Supplementary Figure 3D