1. HA-specific Abs in serum 14 d after a single vaccination with the various targeted DNA vaccines.
HA-specific Abs in serum 14 d after a single vaccination with the various targeted DNA vaccines. Mice were vaccinated with 50 μl of 0.5 μg/μl DNA i.m. in each quadriceps, followed by electroporation of the injection site. Sera obtained 14 d later were analyzed for total IgG (A), IgG1 (B), and IgG2a (C) anti-HA Abs by ELISA (n = 6, bars indicate mean ± SEM, *p < 0.05, **p < 0.01 compared with the nontargeted αNIP-HA group by Mann–Whitney two-tailed t test). Samples with a titer <50 was given an endpoint titer of 1. (D) HA-specific IgG1 versus IgG2a titers were plotted for the various groups. For reasons of clarity, only means are shown. (E) HA-specific IgG1/IgG2a ratio shown for individual mice. Mean ± SEM is indicated. Dotted line indicates IgG1/IgG2a ratio = 1. Groups have been color coded as orange (primarily IgG1), blue (primarily IgG2a), or green (mix of IgG1 and IgG2a).
Ranveig Braathen et al. ImmunoHorizons 2018;2:38-53
Copyright © 2018 The Authors