Presentation is loading. Please wait.

Presentation is loading. Please wait.

Smooth muscle cells cultured from human saphenous vein exhibit increased proliferation, invasion, and mitogen-activated protein kinase activation in vitro.

Published byOwen Foster Modified over 5 years ago

Similar presentations

Presentation on theme: "Smooth muscle cells cultured from human saphenous vein exhibit increased proliferation, invasion, and mitogen-activated protein kinase activation in vitro."— Presentation transcript:

1 Smooth muscle cells cultured from human saphenous vein exhibit increased proliferation, invasion, and mitogen-activated protein kinase activation in vitro compared with paired internal mammary artery cells  Neil A. Turner, PhD, Selina Ho, BSc, Philip Warburton, David J. O’Regan, MD, FRCS, Karen E. Porter, PhD  Journal of Vascular Surgery  Volume 45, Issue 5, Pages (May 2007) DOI: /j.jvs Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

2 Fig 1 Comparison of proliferation rates induced by fetal calf serum (FCS) and p44/42-mitogen-activated protein kinase (MAPK) activation in smooth muscle cells (SMCs) from the saphenous vein (SV) and internal mammary artery (IMA). A, Paired SV-SMCs and IMA-SMC were serum-starved before exposure to medium containing 10% FCS for 7 days. Cells were counted every 2 to 3 days to generate a proliferation profile (n = 6). Filled squares, SV-SMC; open circles, IMA-SMC. B, Mean areas under the proliferation curves (AUC) (arbitrary units) for SV and IMA cells from each patient. *P < .05, n = 6 (paired t-test). C, Whole cell homogenates from serum-starved cells were prepared and immunoblotted for phosphorylation (p-MAPK) and expression (MAPK) of p44/42-MAPK. A representative immunoblot is shown. D, Pooled densitometry data of relative phospho-MAPK levels in paired SV-SMCs and IMA-SMC from 10 patients. *P < .05 (paired ratio t test). Data are presented as means ± standard error of the mean. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

3 Fig 2 Comparison of growth factor-induced proliferation rates of smooth muscle cells (SMCs) from saphenous vein (SV) and internal mammary artery (IMA) Paired SV-SMCs (filled squares) and IMA-SMC (open circles) were serum-starved before exposure to medium containing either (A) 0.4% fetal calf serum (FCS) plus platelet-derived growth factor (PDGF); (B) 1% FCS plus PDGF; (C) 0.4% FCS plus basic fibroblast growth factor (bFGF); or (D) 1% FCS plus bFGF. Cells were counted every 2 to 3 days to generate a proliferation profile (n = 6). Data are presented as means ± standard error of the mean. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

4 Fig 3 Comparison of invasion and migration rates of smooth muscle cells (SMCs) from saphenous vein (SV) and internal mammary artery (IMA). Paired SV-SMCs and IMA-SMC were serum-starved before performing invasion or migration assays towards a platelet-derived growth factor (PDGF)/interleukin-1 (IL-1) or PDGF stimulus, respectively. A, Representative images of the underside of invasion membranes at the end of the experiment (scale bar = 40 μm). B, Quantification of the invasion rates of SV-SMCs and IMA-SMC. Open bars, Control (no chemotactic stimulus); filled bars, PDGF/IL-1 stimulus. **P < .01 for the effect of PDGF/IL-1. †† P < .01 for comparison of SV-SMCs and IMA-SMC (n = 5, paired t test). C, Quantification of the migration rates of SV-SMCs and IMA-SMC performed in parallel with the above invasion assays. Open bars, Control (no chemotactic stimulus); filled bars. PDGF stimulus. ***P < .001, *P < .05 for the effect of PDGF. NS, Not statistically significant for comparison of SV-SMCs and IMA-SMC (n = 5, paired t test). Data are presented as means ± standard error of the mean. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

5 Fig 4 Comparison of matrix metalloproteinase-2 (MMP-2) and MMP-9 secretion from smooth muscle cells (SMCs) from saphenous vein (SV) and internal mammary artery (IMA). Invasion assay supernatants were analyzed for MMP-2 and MMP-9 secretion using gelatin zymography. A, C Representative zymograms of conditioned medium from invasion assays after control (C) or platelet-derived growth factor (PDGF)/interleukin-1 (IL-1) treatment (P + I). Conditioned medium from HT-1080 cells was resolved as a positive control. B and D, Pooled MMP-2 and MMP-9 densitometry data expressed relative to PDGF/IL-1-treated SV-SMC. Open bars, Control (no chemotactic stimulus); filled bars, PDGF/IL-1 stimulus. NS, not statistically significant for comparison of SV-SMCs and IMA-SMC (n = 4, paired ratio t-test). Data presented as means ± standard error of the mean. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

Download ppt "Smooth muscle cells cultured from human saphenous vein exhibit increased proliferation, invasion, and mitogen-activated protein kinase activation in vitro."

Similar presentations

Peter R. Nelson, MD, Shinji Yamamura, MD, K.Craig Kent, MD 

Peter R. Nelson, MD, Shinji Yamamura, MD, K.Craig Kent, MD 
Inhibition of phosphatidylinositol 3-kinase and protein kinase C attenuates extracellular matrix protein-induced vascular smooth muscle cell chemotaxis 

Inhibition of phosphatidylinositol 3-kinase and protein kinase C attenuates extracellular matrix protein-induced vascular smooth muscle cell chemotaxis 
Angiogenic properties of sustained release platelet-rich plasma: Characterization in-vitro and in the ischemic hind limb of the mouse  Shyamal Chandra.

Angiogenic properties of sustained release platelet-rich plasma: Characterization in-vitro and in the ischemic hind limb of the mouse  Shyamal Chandra.
Heparin inhibits thrombin-induced mitogen-activated protein kinase signaling in arterial smooth muscle cells  Ulf Hedin, MD, PhD, Günter Daum, PhD, Alexander.

Heparin inhibits thrombin-induced mitogen-activated protein kinase signaling in arterial smooth muscle cells  Ulf Hedin, MD, PhD, Günter Daum, PhD, Alexander.
Synthetic smooth muscle cell phenotype is associated with increased nicotinamide adenine dinucleotide phosphate oxidase activity: Effect on collagen secretion 

Synthetic smooth muscle cell phenotype is associated with increased nicotinamide adenine dinucleotide phosphate oxidase activity: Effect on collagen secretion 
Regulation of vascular smooth muscle cell expression and function of matrix metalloproteinases is mediated by estrogen and progesterone exposure  Oscar.

Regulation of vascular smooth muscle cell expression and function of matrix metalloproteinases is mediated by estrogen and progesterone exposure  Oscar.
Zinc finger protein 191 deficiency attenuates vascular smooth muscle cell proliferation, migration, and intimal hyperplasia after endovascular arterial.

Zinc finger protein 191 deficiency attenuates vascular smooth muscle cell proliferation, migration, and intimal hyperplasia after endovascular arterial.
Differential effects of orbital and laminar shear stress on endothelial cells  Alan Dardik, MD, PhD, Leiling Chen, MD, Jared Frattini, MD, Hidenori Asada,

Differential effects of orbital and laminar shear stress on endothelial cells  Alan Dardik, MD, PhD, Leiling Chen, MD, Jared Frattini, MD, Hidenori Asada,
Sustained orbital shear stress stimulates smooth muscle cell proliferation via the extracellular signal-regulated protein kinase 1/2 pathway  Hidenori.

Sustained orbital shear stress stimulates smooth muscle cell proliferation via the extracellular signal-regulated protein kinase 1/2 pathway  Hidenori.
Effect of tissue plasminogen activator on vascular smooth muscle cells

Effect of tissue plasminogen activator on vascular smooth muscle cells
Smooth muscle cells from abdominal aortic aneurysms are unique and can independently and synergistically degrade insoluble elastin  Nathan Airhart, MD,

Smooth muscle cells from abdominal aortic aneurysms are unique and can independently and synergistically degrade insoluble elastin  Nathan Airhart, MD,
Shear stress-stimulated endothelial cells induce smooth muscle cell chemotaxis via platelet-derived growth factor-BB and interleukin-1α  Alan Dardik,

Shear stress-stimulated endothelial cells induce smooth muscle cell chemotaxis via platelet-derived growth factor-BB and interleukin-1α  Alan Dardik,
Bertrand Poussier, MD, Alfredo C

Bertrand Poussier, MD, Alfredo C
Transglutaminase type 2 in human abdominal aortic aneurysm is a potential factor in the stabilization of extracellular matrix  Sung Shin, MD, Yong-Pil.

Transglutaminase type 2 in human abdominal aortic aneurysm is a potential factor in the stabilization of extracellular matrix  Sung Shin, MD, Yong-Pil.
Kristopher G. Maier, PhD, Xuan Han, Benjamin Sadowitz, MD, Karen L

Kristopher G. Maier, PhD, Xuan Han, Benjamin Sadowitz, MD, Karen L
Urokinase-induced smooth muscle cell responses require distinct signaling pathways: A role for the epidermal growth factor receptor  Suzanne M. Nicholl,

Urokinase-induced smooth muscle cell responses require distinct signaling pathways: A role for the epidermal growth factor receptor  Suzanne M. Nicholl,
Angiogenic effects of stromal cell-derived factor-1 (SDF-1/CXCL12) variants in vitro and the in vivo expressions of CXCL12 variants and CXCR4 in human.

Angiogenic effects of stromal cell-derived factor-1 (SDF-1/CXCL12) variants in vitro and the in vivo expressions of CXCL12 variants and CXCR4 in human.
Pressure distention compared with pharmacologic relaxation in vein grafting upregulates matrix metalloproteinase-2 and -9  Ada W.Y. Chung, PhD, Pooja.

Pressure distention compared with pharmacologic relaxation in vein grafting upregulates matrix metalloproteinase-2 and -9  Ada W.Y. Chung, PhD, Pooja.
Growth factors and experimental arterial grafts

Growth factors and experimental arterial grafts
Angiogenic properties of human placenta-derived adherent cells and efficacy in hindlimb ischemia  Aleksandar Francki, PhD, Kristen Labazzo, PhD, Shuyang.

Angiogenic properties of human placenta-derived adherent cells and efficacy in hindlimb ischemia  Aleksandar Francki, PhD, Kristen Labazzo, PhD, Shuyang.

Similar presentations

Ads by Google