1. Preclinical Advances with Multiphoton Microscopy in Live Imaging of Skin Cancers 
Thomas Yang Sun, Ann M. Haberman, Valentina Greco 
Journal of Investigative Dermatology 
Volume 137, Issue 2, Pages (February 2017)
DOI: /j.jid
Copyright © 2016 The Authors Terms and Conditions

2. Figure 1
Multiphoton microscopy captures dynamic behaviors of components of the skin microenvironment and tumor cells. (a) Time-lapse images of CX3CR1-GFP–marked immune cell migrating over collagen shown with second harmonic generation. Hair follicle marked by K14CreER;tdTomato is shown in red. Arrow depicts migration vector of the immune cell. (b) Ectopic tumor formed in K14H2BGFP; K19CreER; β-cateninfl(Ex3)/+;tdTomato hair follicle is shown by dashed line. Time-lapse images from square section depict proliferation of wild-type cell (top two boxes) along with proliferation of mutant cell (bottom two boxes). Arrows and arrowheads depict the daughter cells after division. Image adapted from Deschene et al., 2014.
Reprinted with permission from American Association for the Advancement of Science. SHG, second harmonic generation. Scale bar = 20 μm. s, seconds.
Journal of Investigative Dermatology  , DOI: ( /j.jid )
Copyright © 2016 The Authors Terms and Conditions

3. Figure 2
Noninvasive imaging visualizes epithelial and fibroblast cell populations in homeostasis and in tumors. (a) Noninvasive imaging setup using mouse ear as an example. A light, nonadherent glass coverslip is lowered on top of the ear. (b) K14H2BGFP;Lef1RFP cells in a papilloma formed after 6 weeks of DMBA (i.e., 7,12-dimethylbenz[a]anthracene) treatment. (c) In normal homeostasis, z-stack images showing K14H2BGFP suprabasal cells (left), basal cells (middle) and hair follicle (right) nested in collagen. Scale bar = 20 μm. HS, hair shaft; SG, sebaceous gland; HG, hair germ; DP, dermal papilla.
Journal of Investigative Dermatology  , DOI: ( /j.jid )
Copyright © 2016 The Authors Terms and Conditions