1. Fixation and embedding

2. Staining Hematoxylin & Eosin Periodic acid Schiff Toluidine blue
Silver

3. Light microscopy

4. Inverted microscope

5. Phase contrast microscope

6. Differential interference contrast (DIC) component

7. Electron microscope
scanning
transmission

10. Epithelium

15. Connective tissue

16. Glycosaminoglycan

17. Collagen

18. Collagen Type Molecule Structural Features Representative Tissues
Composition
Fibrillar Collagens
I [α1(I)]2[α2(I)] 300-nm-long fibrils Skin, tendon, bone, ligaments,
dentin, interstitial tissues
II [α1(II)] nm-long fibrils Cartilage, vitreous humor
III [α1(III)] nm-long fibrils; Skin, muscle, blood vessels
often with type I
V [α1(V)] nm-long fibrils with Similar to type I; also cell
globular N-terminal cultures, fetal tissues
domain; often with type I
Fibril-Associated Collagens
VI [α1(VI)][α2(VI)] Lateral association with Most interstitial tissues
type I; periodic globular
domains
IX [α1(IX)][α2(IX)] Lateral association with Cartilage, vitreous humor
[α3(IX)] type II; N-terminal
globular domain; bound
glycosaminoglycan
Sheet-Forming Collagens
IV [α1(IV)]2[α2(IV)] Two-dimensional network All basal laminaes

20. Bone

21. Bone marrow

22. Hematopoiesis

27. Neuron

30. Synapse

32. Sarcoplasmic reticulum

33. Take home message
Neuronal structure
Neuromuscular junction