1. Vitrification of human single pronuclear oocytes following two approaches to polar body biopsy 
Ervin Macas, Min Xie, Sara Schaufelberger, Gabriele S. Merki-Feld, Ruth Stiller, Bruno Imthurn 
Reproductive BioMedicine Online 
Volume 22, Issue 4, Pages (April 2011)
DOI: /j.rbmo
Copyright © 2011 Reproductive Healthcare Ltd. Terms and Conditions

2. Figure 1
The mean total cell number (TCN) of blastocysts developed from all three groups of vitrified single pronuclear oocytes and the control group of non-vitrified single pronuclear oocytes. The difference in TCN of blastocysts developed in the laser-treated group and the non-vitrified control was significantly different (P<0.028). PZD=partial zona dissection.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
Copyright © 2011 Reproductive Healthcare Ltd. Terms and Conditions

3. Figure 2
Differential staining of human blastocyst developed from human single pronuclear oocyte. The intense pink colour (propidium iodide and bisbenzimide) represents the chromatin in nuclei of lysed trophectoderm cells. The blue colour (bisbenzimide) represents nuclei of inner cell mass. The arrow depicts the fragmented nucleus of a dead cell. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
Copyright © 2011 Reproductive Healthcare Ltd. Terms and Conditions