Presentation is loading. Please wait.

Presentation is loading. Please wait.

Vascular endothelial growth factor naked DNA gene transfer enhances thrombus recanalization and resolution  Matthew Waltham, MA, PhD, Kevin Burnand, MS,

Published byJoseph Allan Hutchinson Modified over 5 years ago

Similar presentations

Presentation on theme: "Vascular endothelial growth factor naked DNA gene transfer enhances thrombus recanalization and resolution  Matthew Waltham, MA, PhD, Kevin Burnand, MS,"— Presentation transcript:

1 Vascular endothelial growth factor naked DNA gene transfer enhances thrombus recanalization and resolution  Matthew Waltham, MA, PhD, Kevin Burnand, MS, Christiane Fenske, PhD, Bijan Modarai, BSc, MRCS, Julia Humphries, PhD, Alberto Smith, PhD  Journal of Vascular Surgery  Volume 42, Issue 6, Pages (December 2005) DOI: /j.jvs Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

2 Fig 1 Section of psoas muscle adjacent to the vena cava following gene therapy with pCMVβ control plasmid. Myocytes expressing β-galactosidase stained blue (bar = 100 μm). Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

3 Fig 2 Human vascular endothelial growth factor (VEGF) protein levels in muscle after injection of phVEGF or pCMVβ control. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

4 Fig 3 Recanalization during normal and vascular endothelial growth factor (VEGF)-enhanced thrombus resolution. a, Scanning electron micrographs of cross section of naturally resolving 7- day-old thrombus at the level of the silk ligature (S) shows evidence of recanalization by the appearance of endothelial-like cell-lined spaces between the thrombus and vessel wall (b, inset) (bar = 100 μm). c, Transverse section of naturally resolving thrombus shows cell-lined recanalization channels (arrows) around the organizing thrombus by 7 days. These contain erythrocytes (stained yellow) showing that the channels are in continuity with the circulation (bar = 300 μm). d, Higher magnification of thrombus shows cell-lined channels appearing as early as 7 days in areas of organization (collagen deposition, blue stain) within the body of the thrombus. These channels also contain erythrocytes, again suggesting continuity with the circulation (arrows) (bar = 100 μm). e, f, Recanalization channels (arrows) forming in otherwise poorly organized areas of the thrombus contain a high erythrocyte content in animals treated with the phVEGF plasmid (bar = 100 μm). Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

5 Fig 4 Effect of phVEGF or control (pCMVβ) treatment on (a) thrombus size; and (b) area of recanalization expressed as a percentage of vein lumen. Columns show mean and standard error of mean. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

Download ppt "Vascular endothelial growth factor naked DNA gene transfer enhances thrombus recanalization and resolution  Matthew Waltham, MA, PhD, Kevin Burnand, MS,"

Similar presentations

Therapeutic angiogenesis: A comparative study of the angiogenic potential of acidic fibroblast growth factor and heparin  Todd K. Rosengart, MD, Kurt.

Therapeutic angiogenesis: A comparative study of the angiogenic potential of acidic fibroblast growth factor and heparin  Todd K. Rosengart, MD, Kurt.
Liposuction-derived human fat used for vascular graft sodding contains endothelial cells and not mesothelial cells as the major cell type  Stuart K. Williams,

Liposuction-derived human fat used for vascular graft sodding contains endothelial cells and not mesothelial cells as the major cell type  Stuart K. Williams,
Vein wall re-endothelialization after deep vein thrombosis is improved with low- molecular-weight heparin  Daria K. Moaveni, MD, Erin M. Lynch, MS, Cathy.

Vein wall re-endothelialization after deep vein thrombosis is improved with low- molecular-weight heparin  Daria K. Moaveni, MD, Erin M. Lynch, MS, Cathy.
Bioengineered vascular access maintains structural integrity in response to arteriovenous flow and repeated needle puncture  Bryan W. Tillman, MD, PhD,

Bioengineered vascular access maintains structural integrity in response to arteriovenous flow and repeated needle puncture  Bryan W. Tillman, MD, PhD,
Decellularized vein as a potential scaffold for vascular tissue engineering  Patrick J Schaner, MD, Niels D Martin, MD, Thomas N Tulenko, PhD, Irving M.

Decellularized vein as a potential scaffold for vascular tissue engineering  Patrick J Schaner, MD, Niels D Martin, MD, Thomas N Tulenko, PhD, Irving M.
Reduced hind limb ischemia-reperfusion injury in Toll-like receptor-4 mutant mice is associated with decreased neutrophil extracellular traps  Rahmi Oklu,

Reduced hind limb ischemia-reperfusion injury in Toll-like receptor-4 mutant mice is associated with decreased neutrophil extracellular traps  Rahmi Oklu,
In vivo electroporation of constitutively expressed HIF-1α plasmid DNA improves neovascularization in a mouse model of limb ischemia  Geoffrey O. Ouma,

In vivo electroporation of constitutively expressed HIF-1α plasmid DNA improves neovascularization in a mouse model of limb ischemia  Geoffrey O. Ouma,
Vascular endothelial growth factor gene therapy with intramuscular injections of plasmid DNA enhances the survival of random pattern flaps in a rat model 

Vascular endothelial growth factor gene therapy with intramuscular injections of plasmid DNA enhances the survival of random pattern flaps in a rat model 
Yi-kuan Chen, MD, Xue-mei Jiang, MSc, Jian-ping Gong, MD 

Yi-kuan Chen, MD, Xue-mei Jiang, MSc, Jian-ping Gong, MD 
Perivascular innate immune events modulate early murine vein graft adaptations  Binh T. Nguyen, MD, Peng Yu, MD, Ming Tao, MD, Shuai Hao, BS, Tianyu Jiang,

Perivascular innate immune events modulate early murine vein graft adaptations  Binh T. Nguyen, MD, Peng Yu, MD, Ming Tao, MD, Shuai Hao, BS, Tianyu Jiang,
An engineered vascular endothelial growth factor-activating transcription factor induces therapeutic angiogenesis in ApoE knockout mice with hindlimb.

An engineered vascular endothelial growth factor-activating transcription factor induces therapeutic angiogenesis in ApoE knockout mice with hindlimb.
Vascular endothelial growth factor naked DNA gene transfer enhances thrombus recanalization and resolution  Matthew Waltham, MA, PhD, Kevin Burnand, MS,

Vascular endothelial growth factor naked DNA gene transfer enhances thrombus recanalization and resolution  Matthew Waltham, MA, PhD, Kevin Burnand, MS,
Pressure distention compared with pharmacologic relaxation in vein grafting upregulates matrix metalloproteinase-2 and -9  Ada W.Y. Chung, PhD, Pooja.

Pressure distention compared with pharmacologic relaxation in vein grafting upregulates matrix metalloproteinase-2 and -9  Ada W.Y. Chung, PhD, Pooja.
Effect of blocking platelet activation with AZD6140 on development of abdominal aortic aneurysm in a rat aneurysmal model  Jianping Dai, MD, PhD, Liliane.

Effect of blocking platelet activation with AZD6140 on development of abdominal aortic aneurysm in a rat aneurysmal model  Jianping Dai, MD, PhD, Liliane.
Central venous catheter failure is induced by injury and can be prevented by stabilizing the catheter tip  Ted R. Kohler, MD, Thomas R. Kirkman, BS  Journal.

Central venous catheter failure is induced by injury and can be prevented by stabilizing the catheter tip  Ted R. Kohler, MD, Thomas R. Kirkman, BS  Journal.
Adenoviral-mediated expression of antisense RNA to basic fibroblast growth factor reduces tangential stress in arterialized vein grafts  Abigail K. Hanna,

Adenoviral-mediated expression of antisense RNA to basic fibroblast growth factor reduces tangential stress in arterialized vein grafts  Abigail K. Hanna,
Skin erythrodiapedesis during chronic venous disorders

Skin erythrodiapedesis during chronic venous disorders
Treatment of thromboangiitis obliterans (Buerger's disease) by intramuscular gene transfer of vascular endothelial growth factor: Preliminary clinical.

Treatment of thromboangiitis obliterans (Buerger's disease) by intramuscular gene transfer of vascular endothelial growth factor: Preliminary clinical.
Adventitial delivery of platelet-derived endothelial cell growth factor gene prevented intimal hyperplasia of vein graft  Mitsuteru Handa, MD, Wei Li,

Adventitial delivery of platelet-derived endothelial cell growth factor gene prevented intimal hyperplasia of vein graft  Mitsuteru Handa, MD, Wei Li,
Adenovirus-mediated intra-arterial delivery of cellular repressor of E1A-stimulated genes inhibits neointima formation in rabbits after balloon injury 

Adenovirus-mediated intra-arterial delivery of cellular repressor of E1A-stimulated genes inhibits neointima formation in rabbits after balloon injury 

Similar presentations

Ads by Google