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Effect of differential shear stress on platelet aggregation, surface thrombosis, and endothelialization of bilateral carotid-femoral grafts in the dog 

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Presentation on theme: "Effect of differential shear stress on platelet aggregation, surface thrombosis, and endothelialization of bilateral carotid-femoral grafts in the dog "— Presentation transcript:

1 Effect of differential shear stress on platelet aggregation, surface thrombosis, and endothelialization of bilateral carotid-femoral grafts in the dog  Moses Hong-De Wu, MD, Yasuhiro Kouchi, MD, Yoko Onuki, MD, Qun Shi, MD, Hiroki Yoshida, MD, Svetlana Kaplan, PhD, Robert F. Viggers, MS, Rafik Ghali, MD, Lester R. Sauvage, MD  Journal of Vascular Surgery  Volume 22, Issue 4, Pages (October 1995) DOI: /S (95) Copyright © 1995 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

2 Fig. 1 Drawing of surgical model for bilateral carotid-femoral bypasses with arteriovenous (A-V) fistula on one side. Journal of Vascular Surgery  , DOI: ( /S (95) ) Copyright © 1995 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

3 Fig. 2 Comparison of two gross specimens that had been implanted in same dog. A, HSS bypass. B, LSS bypass. (A-V, Arteriovenous; Rt, right; Lt, left.) Journal of Vascular Surgery  , DOI: ( /S (95) ) Copyright © 1995 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

4 Fig. 2 Comparison of two gross specimens that had been implanted in same dog. A, HSS bypass. B, LSS bypass. (A-V, Arteriovenous; Rt, right; Lt, left.) Journal of Vascular Surgery  , DOI: ( /S (95) ) Copyright © 1995 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

5 Fig. 3 Studies used to verify endothelial nature of cells. A, AgNO3 staining shows endothelial-like cell borders. (Original magnification ×45.) B, Positive FVIII/vWF stain on flow surface. (Original magnification ×85.) C, Scanning electron microscopy. (Original magnification ×764.) D, Transmission electron microscopy. (Original magnification ×4070.) Journal of Vascular Surgery  , DOI: ( /S (95) ) Copyright © 1995 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

6 Fig. 3 Studies used to verify endothelial nature of cells. A, AgNO3 staining shows endothelial-like cell borders. (Original magnification ×45.) B, Positive FVIII/vWF stain on flow surface. (Original magnification ×85.) C, Scanning electron microscopy. (Original magnification ×764.) D, Transmission electron microscopy. (Original magnification ×4070.) Journal of Vascular Surgery  , DOI: ( /S (95) ) Copyright © 1995 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

7 Fig. 3 Studies used to verify endothelial nature of cells. A, AgNO3 staining shows endothelial-like cell borders. (Original magnification ×45.) B, Positive FVIII/vWF stain on flow surface. (Original magnification ×85.) C, Scanning electron microscopy. (Original magnification ×764.) D, Transmission electron microscopy. (Original magnification ×4070.) Journal of Vascular Surgery  , DOI: ( /S (95) ) Copyright © 1995 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

8 Fig. 3 Studies used to verify endothelial nature of cells. A, AgNO3 staining shows endothelial-like cell borders. (Original magnification ×45.) B, Positive FVIII/vWF stain on flow surface. (Original magnification ×85.) C, Scanning electron microscopy. (Original magnification ×764.) D, Transmission electron microscopy. (Original magnification ×4070.) Journal of Vascular Surgery  , DOI: ( /S (95) ) Copyright © 1995 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

9 Fig. 4 Comparative thicknesses of neointima and pseudointima. HSS (A and C) and LSS (B and D) grafts show increased thickening of neointima and pseudointima of latter. A and B were implanted in same animal, as were C and D. (Clear areas on lower righthand sides of A and C are spaces previously occupied by supported rings of grafts.) (Hematoxylin and eosin stain. Original magnification ×95.) Journal of Vascular Surgery  , DOI: ( /S (95) ) Copyright © 1995 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

10 Fig. 4 Comparative thicknesses of neointima and pseudointima. HSS (A and C) and LSS (B and D) grafts show increased thickening of neointima and pseudointima of latter. A and B were implanted in same animal, as were C and D. (Clear areas on lower righthand sides of A and C are spaces previously occupied by supported rings of grafts.) (Hematoxylin and eosin stain. Original magnification ×95.) Journal of Vascular Surgery  , DOI: ( /S (95) ) Copyright © 1995 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

11 Fig. 4 Comparative thicknesses of neointima and pseudointima. HSS (A and C) and LSS (B and D) grafts show increased thickening of neointima and pseudointima of latter. A and B were implanted in same animal, as were C and D. (Clear areas on lower righthand sides of A and C are spaces previously occupied by supported rings of grafts.) (Hematoxylin and eosin stain. Original magnification ×95.) Journal of Vascular Surgery  , DOI: ( /S (95) ) Copyright © 1995 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

12 Fig. 4 Comparative thicknesses of neointima and pseudointima. HSS (A and C) and LSS (B and D) grafts show increased thickening of neointima and pseudointima of latter. A and B were implanted in same animal, as were C and D. (Clear areas on lower righthand sides of A and C are spaces previously occupied by supported rings of grafts.) (Hematoxylin and eosin stain. Original magnification ×95.) Journal of Vascular Surgery  , DOI: ( /S (95) ) Copyright © 1995 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

13 Fig. 5 Verification of smooth muscle cells in neointima and external capsule by smooth muscle α-actin staining and demonstration of fewer smooth muscle cells in neointima of HSS (A) than in LSS (B) grafts implanted in same animal. (Original magnification ×100.) Journal of Vascular Surgery  , DOI: ( /S (95) ) Copyright © 1995 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

14 Fig. 5 Verification of smooth muscle cells in neointima and external capsule by smooth muscle α-actin staining and demonstration of fewer smooth muscle cells in neointima of HSS (A) than in LSS (B) grafts implanted in same animal. (Original magnification ×100.) Journal of Vascular Surgery  , DOI: ( /S (95) ) Copyright © 1995 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions


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