1. Polyphenols modulate calcium-independent mechanisms in human arterial tissue- engineered vascular media 
Myriam Diebolt, PhD, Karina Laflamme, PhD, Raymond Labbé, MD, FRCS(C), François A. Auger, MD, FRCP(C), Lucie Germain, PhD, Ramaroson Andriantsitohaina, PhD 
Journal of Vascular Surgery 
Volume 46, Issue 4, Pages (October 2007)
DOI: /j.jvs
Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

2. Fig 1
Concentration-response curves for (A) bradykinin-induced and (B) histamine-induced contraction of arterial tissue-engineered vascular media (TEVM) rings. Concentration-response curves for (C) 3-morpholinosydnonimine (SIN-1) and (D) forskolin-induced relaxation of arterial TEVM rings that were precontracted with histamine (100 μM). Values are means ± standard error of the mean of 10 arterial TEVM experiments.
Journal of Vascular Surgery  , DOI: ( /j.jvs )
Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

3. Fig 2
Concentration-response curves of histamine-induced contraction in arterial tissue-engineered vascular media (TEVM) rings in the presence of (A) the myosin light chain kinase inhibitor, ML-9 (10 μM), (B) the Rho-kinase inhibitor, Y (0.5 μM), and (C) the protein kinase C inhibitor, GF109203X (30 μM). Values are means ± standard error of the mean of five to six arterial TEVM experiments. *P < .5 and *** P < .001 vs control by use of analysis of variance. ML-9, [1-(5-chloronaphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine.
Journal of Vascular Surgery  , DOI: ( /j.jvs )
Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

4. Fig 3
A, Concentration-response curves show histamine-induced contraction in arterial tissue-engineered vascular media (TEVM) rings cultured 24 hours without (control) or with Provinols (10−2 g/L). Values are means ± standard error of the mean (SEM) of five to six arterial TEVM experiments. B, Histogram shows the measure of Ca2+ ratio in arterial TEVM rings incubated 24 hours in the absence (control) or the presence of Provinols (10−2 g/L). Values are means ± SEM of three arterial TEVM experiments. C, Concentration-response curves show histamine-induced contraction in arterial TEVM rings incubated for 24 hours in the presence of 100 μM manganese (III) tetrakis (1-methyl-4-pyridyl) porphyrin pentachloride (MnTMPyP), without (control) or with Provinols treatment (10−2 g/L). Values are means ± SEM of five to six arterial TEVM experiments. ***P < .001 vs control by use of analysis of variance.
Journal of Vascular Surgery  , DOI: ( /j.jvs )
Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

5. Fig 4
A, Histogram shows the percentage of inhibition of histamine-induced contraction in the presence of the Rho-kinase inhibitor, Y (0.5 μM), the phospholipase A2 inhibitor, quinacrine (10 μM), or both inhibitors, in arterial tissue-engineered vascular media (TEVM) rings cultured 24 hours without (control, CTR) or with Provinols (PRO; 10−2 g/L). Values are means ± standard error of the mean of five to six arterial TEVM experiments. **P < .01 vs control by use of unpaired t test. B, Western blot showing the expression of Rho-kinase in arterial TEVM rings cultured 24 hours without (control) or with Provinols (10−2 g/L). Values are representative of three arterial TEVM experiments. C and D, Western blot, with anti-Rho-kinase and anti-Rho A-, B-, C- isoforms antibodies, showing the (C) activity of Rho and (D) the expression of Rho protein under basal and stimulated (histamine, 10−4 M) conditions in arterial TEVM rings cultured 24 hours without (control) or with Provinols (10−2 g/L). Values are representative of three arterial TEVM experiments.
Journal of Vascular Surgery  , DOI: ( /j.jvs )
Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

6. Fig 5
Histogram shows the percentage of inhibition of histamine-induced contraction in the presence of the myosin light chain kinase (MLCK) inhibitor, ML-9 (10 μM; [1-(5-chloronaphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine]), in arterial tissue-engineered vascular media (TEVM) rings cultured 24 hours without (control) or with Provinols (10−2 g/L). Values are means ± standard error of the mean (SEM) of 5 to 6 arterial TEVM. B, Western blot and optical density measurement of the MLCK expression bands of arterial TEVM rings cultured 24 hours without (control) or with Provinols (10−2 g/L). Values are means ± SEM of blotting from five arterial TEVM experiments. **P < .01 vs control by use of unpaired t test.
Journal of Vascular Surgery  , DOI: ( /j.jvs )
Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

7. Fig 6
A, Western blot and optical density measurement of the α-smooth muscle actin expression bands in arterial tissue-engineered vascular media (TEVM) rings cultured 24 hours without (control) or with Provinols (10−2 g/L). Values are mean ± standard error of the mean of blotting from five arterial TEVM experiments. **P < .01 vs control by use of unpaired t test. B, Western blot showing the expression of serum response factor (SRF) in four arterial TEVM rings cultured 24 hours without (control) or with Provinols (10−2 g/L).
Journal of Vascular Surgery  , DOI: ( /j.jvs )
Copyright © 2007 The Society for Vascular Surgery Terms and Conditions