1. Endothelium-dependent vasorelaxations in response to aggregating platelets are impaired in reversed vein grafts 
Kimihiro Komori, MD, Peter Gloviczki, MD, Russell G. Bourchier, MB, ChB, Virginia M. Miller, PhD, Paul M. Vanhoutte, MD, PhD 
Journal of Vascular Surgery 
Volume 12, Issue 2, Pages (August 1990)
DOI: / (90)90102-G
Copyright © 1990 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

2. Fig. 1
A, Light micrograph of a section taken from the mid portion of a rabbit jugular vein that had been grafted into the carotid artery. (6 μm thick section; × 100; Mason trichromatic stain.) The lumen of the graft is at the top of the figure. E denotes endothelial cells; intimal thickening extends to the media (M); A denotes adventitia. B, Scanning electron micrograph of the endothelial surface from the mid portion of a rabbit jugular vein graft (magnification × 1000).
Journal of Vascular Surgery  , DOI: ( / (90)90102-G)
Copyright © 1990 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

3. Fig. 2
Tracing of changes in isometric tension of unoperated jugular veins (control; left) and vein grafts (right) with and without endothelium. Platelets were added to the organ chamber during a contraction evoked by prostaglandin F2α (PGF2α). The platelet concentration is given as number of platelets per cubic millimeter (mm3). Platelets caused relaxation only in unoperated veins (control) with endothelium (p < 0.05). In vein grafts only contractions were observed. Wo, washout.
Journal of Vascular Surgery  , DOI: ( / (90)90102-G)
Copyright © 1990 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

4. Fig. 3
Effects of apyrase (1.0 units/ml) and methiothepin (10−6 mol/L) on platelet-induced relaxations in rings of unoperated (control) jugular veins with endothelium. The concentration of platelets is given as number of platelets per cubic millimeter (mm3). The relaxations were examined during a contraction evoked by prostaglandin F2α. The relaxations are expressed as percent decrease in tension from the contractions evoked by prostaglandin F2α: 1.1 ± 0.2 gm (n = 6). Data are shown as mean ± SEM. Apyrase reduced relaxation (p < 0.05), but methiothepin did not.
Journal of Vascular Surgery  , DOI: ( / (90)90102-G)
Copyright © 1990 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

5. Fig. 4
Cumulative concentration-response curves to ADP in the presence and absence of apyrase (1.0 unit/ml) in jugular veins from control veins and vein grafts during contractions evoked by prostaglandin F2α. The relaxations are expressed as percent decrease in tension from the contractions evoked by prostaglandin F2α (control, 1.03 ± 0.13 g; vein graft, 0.34 ± 0.12 g: n = 6). Data shown as mean ± SEM. In control veins relaxations to ADP were greater in rings with compared to without endothelium (p < 0.05) and these were significantly reduced by apyrase. Relaxations in vein grafts (right panel) were not different from those of control veins without endothelium.
Journal of Vascular Surgery  , DOI: ( / (90)90102-G)
Copyright © 1990 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

6. Fig. 5
A, Cumulative concentration-response curves to A23187 from control jugular veins and vein grafts, during a contraction to prostaglandin F2α (control 0.88 ± 0.23 g; vein graft, 0.32 ± 0.10 g: n = 4). The relaxations are expressed as percent decrease in tension from the contractions to prostaglandin F2α. Data are shown as means ± SEM. The asterisk denotes that the ED50 for A23187-induced relaxations were significantly different in rings with endothelium from control and vein grafts (log molar concentration = − and −7.8 ± 0.2 mol/L; n = 6, respectively). B, Cumulative concentration-response curves to nitric oxide in control jugular veins and vein grafts without endothelium during a contraction evoked by prostaglandin F2α (control, 0.91 ± 0.12 g; vein graft 0.38 ± 0.13 g; n = 6). The relaxations are expressed as percent decrease in tension from the contractions evoked by prostaglandin F2α. Data are shown as means ± SEM. The asterisk denotes that the ED50 for nitric oxide-induced relaxations were significantly different between control and vein grafts (log molar concentration = −5.9 ± 0.2 and −7.2 ± 0.2; n = 6, respectively).
Journal of Vascular Surgery  , DOI: ( / (90)90102-G)
Copyright © 1990 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

7. Fig. 6
Responses to platelets (75,000/mm3) in control jugular veins and vein grafts. The responses are expressed as percentage decrease or increase in tension from the contractions evoked by prostaglandin F2α (control, 1.10 ± 0.2 g; vein graft, 0.33 ± 0.11 g: n = 6). Data are shown as mean ± SEM. (* denotes significance from control with endothelium; + denotes significance from vein graft with endothelium; p < 0.05).
Journal of Vascular Surgery  , DOI: ( / (90)90102-G)
Copyright © 1990 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

8. Fig. 7
Cumulative concentration-response curves to serotonin in vein grafts during a contraction evoked by prostaglandin F2α The contractions are expressed as percent increase in tension from the contractions evoked by prostaglandin F2α (0.31 ± 0.13 g: n = 6). Data are shown as means ± SEM. Methiothepin (10−6 mol/L) but not ketanserine (10−6 mol/L) significantly reduced the maximal tension; p < 0.05).
Journal of Vascular Surgery  , DOI: ( / (90)90102-G)
Copyright © 1990 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions