1. Immunization with Bin1b decreases sperm motility with compromised fertility in rats 
Wenming Xu, Ph.D., Xiaohu Zhang, Ph.D., Wenying Chen, M.Sc., Kin Lam Fok, M.Sc., Dewi Kenneth Rowlands, Ph.D., Yiu-Loon Chui, Ph.D., Hsiao Chang Chan, Ph.D. 
Fertility and Sterility 
Volume 93, Issue 3, Pages e1 (February 2010)
DOI: /j.fertnstert
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Histological examination of Bin1b-immunized epididymis. Representative sections from caput, corpus, and cauda epididymis from Bin1b-immunized rats (A–C) and conjugate-immunized control rats (D–F) stained with hematoxylin and eosin, with normal epididymal morphology in both groups after immunization. Original magnification, ×400 for A–F.
Fertility and Sterility  , e1DOI: ( /j.fertnstert )
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
Presence of high titer of anti-Bin1b antibodies in immunized rats. Comparison of Bin1b antibody titers, measured by ELISA, in sera from Bin1b-immunized and control-immunized male rats. Data shown are absorbance values at 450 nM with the highest serum concentration used (1:1000) for individual animals at different time points in immunized group (bottom) and control group (top).
Fertility and Sterility  , e1DOI: ( /j.fertnstert )
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
Effect of Bin1b immunization on sperm motility. Sperm recovered from successive regions of the epididymides of Bin1b-immunized and control animals were examined for their motility using CASA. Each value represents the mean percentage of 4 rats. ∗P<.05 versus control sperm.
Fertility and Sterility  , e1DOI: ( /j.fertnstert )
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

5. Figure 4
Effect of anti-Bin1b antibodies on sperm capacitation and AR assessed by CTC staining. Adult rat sperm were incubated with serum extracted from Bin1b-immunized and control animals for 5 hours and stained with CTC with the percentage of sperm exhibiting F, B, and AR patterns varying with dilutions (left, 1:100) and (right, 1:250). Each value represents the mean percentage of three rats. ∗P<.05 versus control sperm.
Fertility and Sterility  , e1DOI: ( /j.fertnstert )
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

6. Localization of Bin1b in rat sperm
Localization of Bin1b in rat sperm. Epifluorescent microscope indicating immunostaining pattern of anti-Bin1b antibodies with rat sperm. Immunolocalization employing anti-Bin1b antibodies (1:200) generated by immunization in immunized group revealed localization of the postacrosome compartment of rat sperm (A); (C) Immune sera from the control group failed to show any immunofluorescence localization. The phase-contrast pictures of A, C are B, D, respectively (×400).
Fertility and Sterility  , e1DOI: ( /j.fertnstert )
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions