1. Altered keratin 17 peptide ligands inhibit in vitro proliferation of keratinocytes and T cells isolated from patients with psoriasis 
Zhu Shen, MD, PhD, Ling Chen, MD, Yu-F. Liu, MD, Tian-W. Gao, MD, PhD, Gang Wang, MD, PhD, Xue-L. Fan, MD, PhD, Jian-Y. Fan, MD, Ping-S. Fan, MD, PhD, Chun-Y. Li, MD, PhD, Bin Liu, MD, Yu-P. Dang, MD, Cheng-X. Li, MD, PhD 
Journal of the American Academy of Dermatology 
Volume 54, Issue 6, Pages (June 2006)
DOI: /j.jaad
Copyright © 2006 American Academy of Dermatology, Inc. Terms and Conditions

2. Fig 1
The trimolecular complex of T cell receptor/p-MHC. T cells recognize epitope peptide in association with product of MHC molecule. This occurs through formation of a trimolecular complex, in which the T cell receptor specifically recognizes peptides bound to MHC molecule at surface of APC.
Journal of the American Academy of Dermatology  , DOI: ( /j.jaad )
Copyright © 2006 American Academy of Dermatology, Inc. Terms and Conditions

3. Fig 2
Induction of psoriatic T cell unresponsiveness events by coincubation and prepulse T-cell proliferation assays. 3H-TdR incorporation assay was used to examine T-cell proliferation (A1, B1, C1) and IFN-γ (A2, B2, C2), IL-2 (A3, B3, C3), IL-4 (A4, B4, C4), IL-10 (A5, B5, C5) and TGF-β (A6, B6, C6) expression levels were measured using corresponding release kits. A1-A6, HLA DRB1∗07-positive APCs were cultured in the presence of altered peptide ligands and washed before addition of T cells. B1-B6, HLA DRB1∗07-positive APCs were cultured in the presence of altered peptide ligands with wild-type epitopes and washed before addition of T cells. C1-C6, HLA DRB1∗07-positive APCs were cultured in the presence of altered peptide ligands and washed before addition of T cells with wild-type epitopes.
Journal of the American Academy of Dermatology  , DOI: ( /j.jaad )
Copyright © 2006 American Academy of Dermatology, Inc. Terms and Conditions

4. Fig 3
Suppression of keratinocyte proliferation by T-cell culture supernatant. Keratinocytes, cultured in SFM medium, were supplemented with different ratios of T-cell culture supernatants (A, 1:10; B, 1:100; C, 1:1000). MTT assay was performed to determine cell growth.
Journal of the American Academy of Dermatology  , DOI: ( /j.jaad )
Copyright © 2006 American Academy of Dermatology, Inc. Terms and Conditions

5. Fig 4
A loop working in the pathogenic process of psoriasis. Keratin 17 has ability to stimulate psoriatic T cells, which produce high levels of IFN-γ. It has, moreover, been demonstrated that the expression of keratin 17 can be induced in vitro by IFN-γ. To date, keratin 17 is the only keratin reported to be induced by IFN-γ. Such a vicious loop may work in the pathogenic process of psoriasis.
Journal of the American Academy of Dermatology  , DOI: ( /j.jaad )
Copyright © 2006 American Academy of Dermatology, Inc. Terms and Conditions