1. Age-related changes in the localization of DNA methyltransferases during meiotic maturation in mouse oocytes 
Lu Zhang, M.Sc., Dan-Yu Lu, M.Sc., Wan-Yun Ma, Ph.D., Ying Li, M.D. 
Fertility and Sterility 
Volume 95, Issue 4, Pages e1 (March 2011)
DOI: /j.fertnstert
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Immunofluorescent localization of Dnmts in pubertal and aging mouse oocytes at various stages of meiotic progression. Representative micrographs of IVM oocytes from (A, C, E, G) aging and (B, D, F, H) pubertal mice show the distribution of (A, B) Dnmt1, (C, D) Dnmt3a, (E, F) Dnmt3b and (G, H) Dnmt3L. GV-intact oocytes were matured in vitro for 0 (GV), 3, 5, 7, 9, or 16 hours and then immunostained with specific antibodies. Each antibody was visualized using Quantum dot 585 (yellow), and the DNA was counterstained using Hoechst33342 (blue). Three independent experiments were performed, which used 30 oocytes per group. Arrowheads indicate the misalignment of chromosomes; arrows show the chromatin of the first polar body. Scale bars = 20 μm.
Fertility and Sterility  , e1DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions