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Air force centrifuge
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Centrifuge A machine that spins materials and separates materials of different densities.
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Using a centrifuge to separate out the parts of a cell
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Ultracentrifuge
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Light Microscope Uses light to produce a larger view of an object. Curved lenses bend the light to enlarge the image.
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Label! Arm Base Body tube Clip Coarse Adjustment Eyepiece; ocular lens
Fine Adjustment High Power lens Lamp (Mirror) Lower Power Lens Nose Stage
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Eyepiece; ocular lens Coarse Adjustment Body tube Fine Adjustment Nose Arm High Power lens Lower Power Lens Stage Clip Diaphragm Base Lamp (Mirror)
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Phase-Contrast Microscope
Compound microscope Phase-Contrast Microscope
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Phase-Contrast Microscope
Shows details without staining. Gives a better picture than regular scope.
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Stereo-microscope or Dissecting microscope
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Stereomicroscope Use to study external structures. Same as a dissecting microscope.
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Microtome
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Scanning electron microscope
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Electron Microscope Can magnify 200,000 times! Uses beams of electrons instead of light.
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Toilet Paper x 500
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Deer Tick X 500
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Resolution – the ability of a microscope to show two points that are close together as separate images
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Red Blood Cells
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Microdissection
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Microdissection Dissect cells under a microscope. E.g. remove the nucleus of a cell
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Intracytoplasmic Sperm Injection
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Tissue Culture A way to keep living cells alive while outside the body. Copy a normal environment in a petri dish.
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HeLa cells stained with antibody to actin (green) , vimentin (red) and DNA (blue)
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HeLa cells Henrietta Lacks
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The cells were propagated by George Otto Gey shortly before Lacks died of her cancer in This was the first human cell line to prove successful in vitro, which was a scientific achievement with profound future benefit to medical research. Gey freely donated both the cells and the tools and processes his lab developed to any scientist requesting them, simply for the benefit of science. Neither Lacks nor her family gave Lacks's physician permission to harvest the cells, but, at that time, permission was neither required nor customarily sought. The cells were later commercialized, although never patented in their original form. Then, as now, there was no requirement to inform a patient, or their relatives, about such matters because discarded material, or material obtained during surgery, diagnosis, or therapy, was the property of the physician and/or medical institution (this currently requires ethical approval and patient consent in the UK). This issue and Lacks's situation was brought up in the Supreme Court of California case of Moore v. Regents of the University of California. The court ruled that a person's discarded tissue and cells are not their property and can be commercialized.
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Embryonic Stem Cells in Culture
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Chromatography Done with special paper. Separates a mixture of chemicals based on their affinity for (attraction to) the paper.
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Electrophoresis Method that separates proteins or DNA fragments. Done with a special gel & electricity.
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Computers MRI, CAT scan, PET scan, Xray
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X- ray
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CAT Scan
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MRI
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PET Scans
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