1. Cytoskeleton and integrin-mediated adhesion signaling in human CD34+ hemopoietic progenitor cells 
Jean-Pierre Lévesque , Paul J. Simmons 
Experimental Hematology 
Volume 27, Issue 4, Pages (April 1999)
DOI: /S X(98)

2. Figure 1
Organization of F-actin in bone marrow stroma fibroblasts (A) and bone marrow CD34+ HPCs (B) after attachment to fibronectin. Cells were adhered for 30 minutes at 37°C on fibronectin-coated glass slides after stimulation with IL-1, IL-3, IL-6, granuloctye colony-stimulating factor, granulocyte-macrophage colony-stimulating factor, and stem cell factor at 10 ng/mL each. After fixation and permeabilization, cells were stained with rhodamine-conjugated phalloidin. Stromal cells were magnified 400×, whereas HPCs were magnified 600×
Experimental Hematology  , DOI: ( /S X(98) )

3. Figure 2
Model of focal adhesion complex organization in spontaneously adherent cells according to Burridge and Chrzanowska-Wodnicka [13] and Lo et al. [42]
Experimental Hematology  , DOI: ( /S X(98) )

4. Figure 3
Bone marrow CD34+ HPCs spread on β1 integrin-mediated attachment to fibronectin. Cells were adhered onto fibronectin-coated (A) and P-selectin–coated (B) wells for 30 minutes at 37°C after stimulation with IL-1, IL-3, IL-6, granuloctye colony-stimulating factor, granulocyte-macrophage colony-stimulating factor, and stem cell factor at 10 ng/mL each. After extensive washes, adherent cells were fixed and processed for transmission electron microscopy (magnification ×6000)
Experimental Hematology  , DOI: ( /S X(98) )

5. Figure 3
Bone marrow CD34+ HPCs spread on β1 integrin-mediated attachment to fibronectin. Cells were adhered onto fibronectin-coated (A) and P-selectin–coated (B) wells for 30 minutes at 37°C after stimulation with IL-1, IL-3, IL-6, granuloctye colony-stimulating factor, granulocyte-macrophage colony-stimulating factor, and stem cell factor at 10 ng/mL each. After extensive washes, adherent cells were fixed and processed for transmission electron microscopy (magnification ×6000)
Experimental Hematology  , DOI: ( /S X(98) )

6. Figure 4
β1 integrins (A) , talin (B), α-actinin (C), and vinculin (D) cluster in FACs in bone marrow CD34+ HPCs. Cells were adhered onto fibronectin-coated glass slides for 30 minutes at 37°C after stimulation with IL-1, IL-3, IL-6, granuloctye colony-stimulating factor, granulocyte-macrophage colony-stimulating factor, and stem cell factor at 10 ng/mL each. After extensive washes, adherent cells were fixed and permeabilized before staining with monoclonal antibodies specific for human β1 integrin subunit, talin, α-actinin, vinculin, and FITC-conjugated donkey anti-mouse IgG immunoglobulins
Experimental Hematology  , DOI: ( /S X(98) )

7. Figure 5
Expression of cytoskeleton components and kinases in bone marrow CD34+ HPCs and in MO7e, TF1, and UT7 myeloid cell lines. Cell lysates representative of 5 × 105 cells were loaded on a 8% polyacrylamide gel in reducing conditions and were analyzed by Western blotting with monoclonal antibodies or rabbit antisera specific for the specified proteins
Experimental Hematology  , DOI: ( /S X(98) )