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Wall shear modulation of cytokines in early vein grafts

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1 Wall shear modulation of cytokines in early vein grafts
Zhihua Jiang, MD, PhD, Scott A Berceli, MD, PhD, Chun L Pfahnl, MA, Lizhen Wu, RN, Darin Goldman, BS, Ming Tao, MD, Motoko Kagayama, MS, Akihiro Matsukawa, MD, C.Keith Ozaki, MD  Journal of Vascular Surgery  Volume 40, Issue 2, Pages (August 2004) DOI: /j.jvs

2 Fig 1 Examples of low (A) and high (B) wall shear vein grafts (28 days; Masson trichrome), and normal jugular vein (C). Journal of Vascular Surgery  , DOI: ( /j.jvs )

3 Fig 2 Relative messenger RNA expression calibrated to normal vein. Results are expressed as relative interleukin-1β or interleukin-10 mRNA expression after normalization to 18S ribosomal RNA, to minimize variability from RNA quality and RT efficiency, and finally calibrated to unmanipulated jugular vein. With arterialization, vein wall interleukin-1β exhibits rapid induction that is higher in the low shear stress setting. On the other hand, interleukin-10 exhibits relatively delayed upregulation, most notably by high wall shear stress at 14 days. Journal of Vascular Surgery  , DOI: ( /j.jvs )

4 Fig 3 Interleukin-1β localized to developing neointima 3 days after vein graft placement. Unmanipulated control vein demonstrates negative staining. Journal of Vascular Surgery  , DOI: ( /j.jvs )


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