1. Quantitative Analysis of Activating Alpha Subunit of the G Protein (Gsα) Mutation by Pyrosequencing in Fibrous Dysplasia and Other Bone Lesions 
Qi Liang, Minqi Wei, LeAnn Hodge, Julie C. Fanburg-Smith, Ann Nelson, Markku Miettinen, Robert D. Foss, Guanghua Wang 
The Journal of Molecular Diagnostics 
Volume 13, Issue 2, Pages (March 2011)
DOI: /j.jmoldx
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2. Figure 1
Pyrosequencing assay design. PCR amplifies an 86-bp Gsα region containing the codon for Arg201.
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
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3. Figure 2
Quantitative pyrosequencing analysis of Arg201 on PCR products of premixed varied ratio of mutant (Arg→His or CGT→CAT) with wild-type species. A: Pyrosequencing results of mixtures of premixture of mutant and wild-type oligos. B: Correlation of calculate mutation ratio in premixture with that of the detected ratio. Ratio percentage indicated the percentage of mutant species in the overall premixture before PCR reactions. Data were obtained from three experiments.
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4. Figure 3
Typical pyrogram of wild-type, Arg(CGT)→Cys(TGT), and Arg(CGT)→His(CAT) mutations as observed in fibrous dysplasia cases. Case #8429, with low mutant content, can be readily determined compared with wild-type case #8205. Red asterisks indicate mutant peaks.
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5. Figure 4
Typical sequencing patterns of wild-type, Arg(CGT)→Cys(TGT), and Arg(CGT)→His(CAT) mutations as observed in fibrous dysplasia cases. Down-pointing arrows indicate mutation sites in the genetic codon CGT for Arg. Note that mutant content <20% might be difficult to determine, as in the case 8429 with 11% mutant determined by pyrosequencing in Figure 3.
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6. Figure 5
Examples of histological feature on H&E of some FD and BFOL cases. A: Fibrous dysplasia with mutation. B: Rare FD without evidence of mutation. C: BFOL not otherwise specified, with mutation (probably fibrous dysplasia). D: Ossifying fibroma from the jaw, negative for mutation.
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
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