1. Successful pregnancies after transplantation of frozen–thawed mouse ovaries into chimeric mice that received lethal-dose radiation 
Fujio Migishima, M.D., Ph.D., Rika Suzuki-Migishima, B.A., Rudolfo B. Quintero, M.D., Minesuke Yokoyama, Ph.D., Barry R. Behr, Ph.D., H.C.L.D. 
Fertility and Sterility 
Volume 86, Issue 4, Pages (October 2006)
DOI: /j.fertnstert
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

2. FIGURE 1
Flow-cytometric analysis of peripheral blood nucleated cells from C57BL/6 mice, GFP-transgenic mice, and radiation-chimeric mice transfused with GFP+ umbilical cord blood. Bars, GFP+ cells.
Migishima. Pregnancy after lethal dose irradiation. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

3. FIGURE 2
Staining PBLs with antibodies to different lineage markers revealed that transfused cells had developed into T cells, B cells, Mac-1+ cells, granulocytes, and erythrocytes. Every lineage contained GFP+ cells.
Migishima. Pregnancy after lethal dose irradiation. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

4. FIGURE 3
Abdominal cavity of 18.5-day-pregnant chimeric mouse that was transplanted with frozen–thawed ovaries. (A) Observation under visible light. (B) Observation when visible light and GFP excitation light are simultaneously used for illumination. F = fetus of GFP-transgenic mouse; L = lymph node of chimeric mouse (GFP fluorescence was observed in lymph node); O = transplanted frozen–thawed ovary from GFP-transgenic mouse (+/+); P = Peyer’s patchlike tissue on colon, where GFP fluorescence was also observed; K = kidney; T = ovarian tube; U = part of the uterus. The GFP fluorescence was not observed in the kidney, ovarian tube, and uterus of the recipient.
Migishima. Pregnancy after lethal dose irradiation. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

5. FIGURE 4
Histology of ovaries from lethally irradiated recipients that were transfused with GFP+ UCBCs. (A) Whole ovary from the radiation-chimeric mouse. Follicles were not observed in the ovarian tissue from irradiated mice. Cells that were GFP+ were noted sporadically in the ovary from the irradiated mouse. (B) Ovarian cortex from irradiated mouse. Follicles were not observed in the ovarian cortex from the irradiated mouse. (C) Medulla of the ovary from a chimeric mouse. Considerable large GFP+ cells were detected in panels B and C. (D) Merged images of considerable large GFP+ cells stained with anti-CD 45 antibody–labeled biotin and Texas red–labeled streptavidin. The GFP+ cells were considered to be macrophages by morphological findings. In every panel, bar = 100 μm.
Migishima. Pregnancy after lethal dose irradiation. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

6. FIGURE 5
Stereomicroscopic findings with 480-nm blue light and with visible light of the recipients and donor. (A–C) Fresh ovarian grafts of GFP-transgenic mouse 4 months after transplantation. (D–F) Frozen–thawed ovarian grafts of GFP-transgenic mouse 4 months after transplantation. Note that the size of the fresh ovaries is generally larger than that of the frozen–thawed ovaries. (A, D) Findings with visible light. (B, E) Findings with 480-nm blue light. (C, F) Merged findings with 480-nm blue light and visible light. In every panel, bar = 200μm.
Migishima. Pregnancy after lethal dose irradiation. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions