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High-Efficiency Stable Gene Transduction in Primary Human Melanocytes Using a Lentiviral Expression System Shariff Dunlap, Xiaobing Yu, Linzhao Cheng, Curt I. Civin, Rhoda M. Alani Journal of Investigative Dermatology Volume 122, Issue 2, Pages (February 2004) DOI: /j X x Copyright © 2004 The Society for Investigative Dermatology, Inc Terms and Conditions
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Figure 1 Primary human melanocytes maintain high-level expression of transduced genes several weeks after lentiviral infection. (A) Phase contrast and fluorescence images of primary human melanocytes infected with EF. GFP lentivirus 48 h after infection. Note all cells seen phase contrast express GFP. (B) Phase contrast and fluorescence images of primary human melanocytes infected with EF-GFP lentivirus 4 wk after infection. Note all cells seen in phase contrast express GFP. Journal of Investigative Dermatology , DOI: ( /j X x) Copyright © 2004 The Society for Investigative Dermatology, Inc Terms and Conditions
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Figure 2 High-efficiency gene transduction in primary human melanocytes using a bicistronic lentivirus. Phase contrast image (A) and fluorescence microscopy of primary human melanocytes infected with a bicistronic lentivirus expressing GFP (B) and RFP (C) at 24 h after infection. Note expression of GFP in uninfected (–) and infected (+) cells as determined by western analysis 72 h after infection (D). Journal of Investigative Dermatology , DOI: ( /j X x) Copyright © 2004 The Society for Investigative Dermatology, Inc Terms and Conditions
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