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Immunofluorescence staining of virus-infected human placental explants

Published byYandi Hermawan Modified over 5 years ago

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Presentation on theme: "Immunofluorescence staining of virus-infected human placental explants"— Presentation transcript:

1 Fig. 6 Immunofluorescence staining of virus-infected human placental explants.
Immunofluorescence staining of virus-infected human placental explants. Confocal micrographs of human placental tissues (chorionic villi, decidua, and fetal membrane) from four donors (16 to 23 weeks of gestation) that were inoculated with the indicated viruses [mock (PBS), ZIKV, WNV, POWV, CHIKV, and MAYV] for 48 hours. Immunofluorescence microscopy was performed for vRNA [using anti-dsRNA (double-stranded RNA) antibody; green] and tissue counterstained for cytokeratin-19 (Cyt-19; a marker of epithelial-derived cells, including trophoblasts) or actin (in red, as indicated below each panel). DAPI (4′,6-diamidino-2-phenylindole)–stained nuclei are shown in blue. White boxes denote zoomed regions shown at bottom right. Images are representative of four explants per experiment from four separate donors under each condition. Scale bar, 50 μm. Derek J. Platt et al., Sci Transl Med 2018;10:eaao7090 Published by AAAS

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