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Volume 111, Issue 6, Pages (September 2016)

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1 Volume 111, Issue 6, Pages 1173-1179 (September 2016)
Identification of HIV-1–Based Virus-like Particles by Multifrequency Atomic Force Microscopy  Irene González-Domínguez, Sonia Gutiérrez-Granados, Laura Cervera, Francesc Gòdia, Neus Domingo  Biophysical Journal  Volume 111, Issue 6, Pages (September 2016) DOI: /j.bpj Copyright © 2016 Biophysical Society Terms and Conditions

2 Figure 1 EM analysis of HIV-1 Gag VLP preparations. (a) TEM analysis. (b) SEM analysis, from deposits on carbon-coated grids. (Straight blue arrows) HIV-1 Gag VLPs; (red dashed arrows) cellular vesicle structures. To see this figure in color, go online. Biophysical Journal  , DOI: ( /j.bpj ) Copyright © 2016 Biophysical Society Terms and Conditions

3 Figure 2 TEM and AFM imaging of an individual HIV-1 Gag VLP. (a) TEM image. (b) AFM 3D topography imaging. (c) AFM amplitude and (d) phase image of the fundamental mode. (e) Second amplitude (A2) and (f) phase (Φ2) images of bimodal AFM, corresponding to the dynamics of the first excited eigenmode. (g) First excited eigenmode frequency shift (Δf2) image of AM-FM viscoelastic mapping mode, and the corresponding calculated (h) Young’s modulus image. To see this figure in color, go online. Biophysical Journal  , DOI: ( /j.bpj ) Copyright © 2016 Biophysical Society Terms and Conditions

4 Figure 3 Individual VLP deposited on mica substrate. (a) AFM topography image of VLPs on mica substrate. (b) 3D topography image of a single VLP structure. (c) Δf2 imaging of an isolated VLP structure, as obtained by AM-FM viscoelastic mapping mode and (d) the corresponding calculated Young’s modulus distribution. To see this figure in color, go online. Biophysical Journal  , DOI: ( /j.bpj ) Copyright © 2016 Biophysical Society Terms and Conditions

5 Figure 4 “Mock” sample deposited on mica substrate. (a) AFM topography image of empty cellular vesicles deposited on mica substrate; (b) corresponding AFM phase image, showing the contrast due to the presence of vesicles. To see this figure in color, go online. Biophysical Journal  , DOI: ( /j.bpj ) Copyright © 2016 Biophysical Society Terms and Conditions

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