1. H3 HA amino acid mutations that were selected in indicated clades.
H3 HA amino acid mutations that were selected in indicated clades. X-ray diffraction crystal structures of the A/Aichi/02/1968 HA trimer (PDB accession no. 2YPG) visualized using MacPyMol v (A) Mutations that characterized clade 7 (light orange), clade 3B (pale cyan), and clade 3C (marine) are indicated. (B) Mutations that characterize clade 3C.3a (salmon), 3C.2a (brick), or both (violet) are indicated. Antigenic sites are colored shades of green as follows: A (pale), B (smudge), C (limon), D (forest), E (split pea). Lines connect residue text with their corresponding amino acids. Residue mutations are shown in black, and estimated ΔΔG values are in gray. Mutations that altered a putative glycosylation site are noted in red if gained and blue if lost. The areas of the circles are proportional to the estimated ΔΔG values. Closed circles indicate an increase in stability, and open circles indicate a decrease in stability.
Eili Y. Klein et al. mSphere 2018; doi: /mSphereDirect