1. Increased expression of p21-activated kinase 4 in adenomyosis and its regulation of matrix metalloproteinase-2 and -9 in endometrial cells 
Kyong Wook Yi, M.D., Ph.D., Sung Hoon Kim, M.D., Ph.D., Hyo Jin Ihm, M.S., Young Sang Oh, M.S., Hee Dong Chae, M.D., Ph.D., Chung-Hoon Kim, M.D., Ph.D., Byung Moon Kang, M.D., Ph.D. 
Fertility and Sterility 
Volume 103, Issue 4, Pages e2 (April 2015)
DOI: /j.fertnstert
Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Representative micrographs (×400) of Pak4 immunostaining in the eutopic endometrium of (A, left) control subjects, (A, middle) eutopic endometrium of adenomyosis patients, and (A, right) adenomyosis nodule of adenomyosis patients; and HSCORES of the (B, left) glandular cells and the (B, right) stromal cells. ES = early secretory phase; LS = late secretory phase; MS = midsecretory phase; P = proliferative phase; S = secretory phase; W = all endometrial dates combined. Data for HSCORES are expressed as mean ± SEM. ∗, P<.005 vs. control; †, P<.001 vs. control.
Fertility and Sterility  , e2DOI: ( /j.fertnstert )
Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
Effect of Pak4 expression on MMP-2 and -9 activities in (A) Ishikawa cells and (B) ESCs. Western blot analysis for Pak4 (left) and zymography for MMP-2 (middle) and MMP-9 (right) in Ishikawa cells (n = 5) and ESCs (n = 7) after transfection with control siRNA and Pak4 siRNA. Data are expressed as a percentage, wherein cells transfected with control siRNA are normalized to 100%. ∗, P<.05 vs. cells transfected with control siRNA.
Fertility and Sterility  , e2DOI: ( /j.fertnstert )
Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
Pak4 expression following TNF-α and PDTC treatment in endometrial cells. NF-κB p65-DNA binding immunodetection assay in (A, left) Ishikawa cells (n = 5) and (B, left) ESCs (n = 5) treated with either vehicle (C), 10 ng/mL TNF-α (T10), or 10 ng/mL TNF-α with 0.5 μM PDTC (P0.5). Western blot analysis for Pak4 in (A, right) Ishikawa cells (n = 5) and (B, right) ESCs (n = 5) treated with either vehicle (C), 10 ng/mL TNF-α (T10), or 10 ng/mL TNF-α with 0.5 μM PDTC (P0.5). Data are expressed as a percentage, wherein cells treated with the vehicle are normalized to 100%. ∗, P<.05 vs. vehicle. †, P<.05 vs. cells treated with 10 ng/mL TNF-α.
Fertility and Sterility  , e2DOI: ( /j.fertnstert )
Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

5. Supplemental Figure 1
Expression of lamin B in (A) Ishikawa cells and (B) ESCs after nuclear protein extraction. C = cytoplasmic fraction; N = nuclear fraction.
Fertility and Sterility  , e2DOI: ( /j.fertnstert )
Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

6. Supplemental Figure 2
Expression of NF-κB p65 subunit following TNF-α and PDTC treatment in the nuclear fraction of (A) Ishikawa cells and (B) ESCs. C = vehicle; T = TNF-α; T+P = TNF-α + PDTC.
Fertility and Sterility  , e2DOI: ( /j.fertnstert )
Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions