1. Volume 149, Issue 7, Pages 1910-1919.e5 (December 2015)
Yarrowia lipolytica Lipase 2 Is Stable and Highly Active in Test Meals and Increases Fat Absorption in an Animal Model of Pancreatic Exocrine Insufficiency 
Ahmed Aloulou, Mathieu Schué, Delphine Puccinelli, Stéphane Milano, Chantal Delchambre, Yves Leblond, René Laugier, Frédéric Carrière 
Gastroenterology 
Volume 149, Issue 7, Pages e5 (December 2015)
DOI: /j.gastro
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2. Figure 1
Variations with pH in the specific activities of YLLIP2, rDGL, HGL, rHPL, and PPL on long-chain TAG under optimized assay conditions. The YLLIP2 and pancreatic lipases (rHPL and PPL) activities were measured with an olive oil and gum arabic emulsion substrate. Gastric lipases (rDGL and HGL) activities were measured with Intralipid substrate. Values are mean ± SD (n = 3). nDGL, native dog gastric lipase.
Gastroenterology  , e5DOI: ( /j.gastro )
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3. Figure 2
In vitro lipolysis of test meal TAG by YLLIP2, rDGL, and rHPL without (A) or with (B) bile. The lipolysis levels at various pH values and after 60 minutes of incubation were estimated from the complete analysis of the lipolysis products. Values are mean ± SD (n = 3).
Gastroenterology  , e5DOI: ( /j.gastro )
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4. Figure 3
Time-course stability of YLLIP2 at various pH levels during test meal TAG lipolysis with and without pepsin. The inserts in (A) and (D) show the immunoreactivity of YLLIP2 at various time points. The experiments were performed without bile. Values are mean ± SD (n = 3).
Gastroenterology  , e5DOI: ( /j.gastro )
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5. Figure 4
Dose-dependent times for the completion of meal TAG lipolysis (minutes). The theoretical duration for the completion of meal TAG lipolysis (ie, the total conversion of meal TAG into FFA and MAG) was estimated based on the amounts of YLLIP2 or pancreatin to be administered to patients, the amounts of TAG in the meal (30 g; 34 mmol), and the specific activities of YLLIP2 or pancreatin on meal TAG (Table 2) assuming a linear release of FFA with time and a constant pH value (4.0 or 6.0). The circles indicate the doses of pancreatin corresponding to 1 (300 mg), 2 (600 mg), 3 (900 mg), or 4 (1,200 mg) capsules of PPE 25,000 U. The arrows indicate the doses (mg) of YLLIP2 that would be required to achieve the digestion of meal TAG at the same rate as 4 PPE capsules at pH 6 (solid line: 8.5 mg YLLIP2) and 4 (dotted line: 15 mg YLLIP2).
Gastroenterology  , e5DOI: ( /j.gastro )
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6. Supplementary Figure 1
In vitro lipolysis of test meal TAG by YLLIP2, rDGL, and rHPL. Apparent specific activities (U/mg of enzyme) at various pH values were estimated from the release of FFA during the first 5 minutes of incubation, in the absence (A) and presence (B) of bile. Values are means ± SD (n = 3).
Gastroenterology  , e5DOI: ( /j.gastro )
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7. Supplementary Figure 2
Time-course hydrolysis of test meal TAG by rHPL (A), YLLIP2 (B), and rDGL (C) at pH 6 and 37°C in the presence of bile. Glyceride species (TAG, DAG, MAG, and calculated glycerol) are expressed as glycerol mole percentages and plotted as a function of the percentage rate of hydrolysis.
Gastroenterology  , e5DOI: ( /j.gastro )
Copyright © 2015 AGA Institute Terms and Conditions