1. Beclin‐1 depletion reduces targeting of several outer kinetochore proteins.
Beclin‐1 depletion reduces targeting of several outer kinetochore proteins. Control and Beclin‐1‐depleted cells were treated with nocodazole before fixation, and labelled with the indicated antibody. (A) CENP‐E and CREST. Two asterisks indicate significant results, P= (B) CENP‐F and CREST. Three asterisks indicate significant results, P= (C) ZW10 and CREST. Three asterisks indicate significant results, P= (D) MAD2 and CREST. Two asterisks indicate significant results, P= (E) HEC1 and CREST. (F) KNL‐1 and CREST. On the right, magnified areas and quantification of the fluorescence intensity of the protein of interest normalized to the fluorescence intensity of CREST staining are shown. Graphs represent three independent experiments (error bars=s.d.). Scale bar, 10 μm. (G) Western blot of whole‐cell lysates from control and Beclin‐1 siRNA‐treated cells. siRNA, small interfering RNA.
Stéphane Frémont et al. EMBO Rep. 2013;14:
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