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Antibody-encoding repertoires of bone marrow and peripheral blood—a focus on IgE  Mattias Levin, PhD, Fredrik Levander, PhD, Robert Palmason, MD, Lennart.

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Presentation on theme: "Antibody-encoding repertoires of bone marrow and peripheral blood—a focus on IgE  Mattias Levin, PhD, Fredrik Levander, PhD, Robert Palmason, MD, Lennart."— Presentation transcript:

1 Antibody-encoding repertoires of bone marrow and peripheral blood—a focus on IgE 
Mattias Levin, PhD, Fredrik Levander, PhD, Robert Palmason, MD, Lennart Greiff, PhD, MD, Mats Ohlin, PhD  Journal of Allergy and Clinical Immunology  Volume 139, Issue 3, Pages (March 2017) DOI: /j.jaci Copyright © 2016 The Authors Terms and Conditions

2 Fig 1 IgE repertoires encoded by cells in BM are more diverse than those encoded by cells in PB. IGHV germline genes origins of IgE-encoding transcripts (present at >0.1%) found in duplicate samples of BM (A) and PB (B) of 6 donors. Hierarchical clustering (C) of V gene usage percentages of 70 genes (Euclidean distances, average linkage) of duplicate samples encoding different isotypes (top boxes: IgA, black; IgE, orange; IgG, green; IgM, red) in different tissues (middle boxes: PB, orange; BM, blue) as derived from different donors (bottom boxes). Duplicate samples of IgA, IgG, and IgM cocluster, and in several cases samples of these isotypes as derived from 1 donor cocluster. In contrast, duplicate samples representing the IgE-encoding repertoire derived from PB, and to some extent BM, do not cluster together. Number of clones encoding different isotypes identified in 10-mL samples of BM (D) and PB (E). Each value represents the mean ± 1 SD of 2 duplicate biological samples collected simultaneously. PB samples from donor 6 did not contain IgE-encoding transcripts and IgG4-encoding transcripts were not identified in samples from donor 5. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 The Authors Terms and Conditions

3 Fig 2 Frequency of transcripts of IgA, IgE, IgG, and IgM encoded by BM and PB and found as related members in duplicate samples of the other tissue (PB and BM, respectively) and in other isotypes of the same tissue type. The analysis included up to 1000 of the commonest transcript variants of each sample (a variant was defined as sequence originating from the same germline gene irrespective of allelic variant and with identical CDRH3-encoding nucleotide sequences; each analyzed sequence set must be larger than 0.01% of the largest sequence set in the sample, and it must be represented by at least 2 sequence reads). Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 The Authors Terms and Conditions

4 Fig E1 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 The Authors Terms and Conditions

5 Fig E2 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 The Authors Terms and Conditions

6 Fig E4 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 The Authors Terms and Conditions

7 Fig E6 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 The Authors Terms and Conditions

8 Fig E7 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 The Authors Terms and Conditions

9 Fig E8 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 The Authors Terms and Conditions

10 Fig E11 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 The Authors Terms and Conditions

11 Fig E12 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 The Authors Terms and Conditions

12 Fig E13 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 The Authors Terms and Conditions

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