Presentation is loading. Please wait.

Presentation is loading. Please wait.

Allergen-induced, eotaxin-rich, proangiogenic bone marrow progenitors: A blood-borne cellular envoy for lung eosinophilia  Kewal Asosingh, PhD, Jodi D.

Published byClyde Powell Modified over 6 years ago

Similar presentations

Presentation on theme: "Allergen-induced, eotaxin-rich, proangiogenic bone marrow progenitors: A blood-borne cellular envoy for lung eosinophilia  Kewal Asosingh, PhD, Jodi D."— Presentation transcript:

1 Allergen-induced, eotaxin-rich, proangiogenic bone marrow progenitors: A blood-borne cellular envoy for lung eosinophilia  Kewal Asosingh, PhD, Jodi D. Hanson, MS, Georgiana Cheng, MD, Mark A. Aronica, MD, Serpil C. Erzurum, MD  Journal of Allergy and Clinical Immunology  Volume 125, Issue 4, Pages (April 2010) DOI: /j.jaci Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Expression of eotaxin by bone marrow–derived EPCs. A, EPCs were isolated from the bone marrow and characterized for the expression of endothelial, progenitor cell, and myeloid markers. Dotted lines indicate autofluorescence and background staining. B, Eotaxin-1 expression by EPCs in different experimental conditions. Bar = 200 μm. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Secretion of eotaxin-1 in cocultures of EPCs and lung vascular cells. A, Isolation of ECs and SMCs from lungs. Dot plots of CD31+ cells before and after purification are shown. Histograms for α-SMC actin (solid line) and calponin (solid line) expression in SMCs are shown. Dashed lines indicate staining with control antibodies. Phase-contrast images of the ECs and SMCs are also shown. Eotaxin-1 secretion by EPCs (B), lung ECs (C), and lung SMCs (D) and cocultures of EPCs with lung ECs (E), EPCs with lung SMCs (F), and EPCs with heart and liver vascular cells (G) are shown. Data represent mean ± SE values of 6 mice in each group. IP, Intraperitoneal. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 Recruitment of eosinophils into the lungs by EPC-conditioned medium. EPC-conditioned supernatants (eotaxin-1 concentration of approximately 400 pg/mL) were intranasally administered into mice that had undergone sham (−) or OVA intraperitoneal (IP) sensitization. Eosinophils in the peripheral blood circulation and recruitment into lungs were analyzed by means of flow cytometry. Preincubation of the conditioned medium with neutralizing eotaxin-1 antibody was performed to block eotaxin-1. Isotype-matched control antibodies had no significant effects (data not shown). Data represent mean ± SE values from 3 mice in each group. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 Eotaxin-1–positive EPCs in allergen-exposed lungs. A, Mononuclear cells were isolated from lungs and evaluated for eotaxin-1 expression. Representative values for 3 mice in each group are shown. B, Eotaxin-1 content per positive cells quantified with Image Pro software. IP, Intraperitoneal. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig 5 Expression of eotaxin-1 by circulating human EPCs. A, Eotaxin-1 expression analyzed by means of immunofluorescence staining. Bar = 200 μm. B, Percentage of eotaxin-1–positive EPCs. C, Eotaxin-1 content per positive cell was quantified with Image Pro software. Median values and upper and lower quartiles are shown. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Download ppt "Allergen-induced, eotaxin-rich, proangiogenic bone marrow progenitors: A blood-borne cellular envoy for lung eosinophilia  Kewal Asosingh, PhD, Jodi D."

Similar presentations

Kyoung-Bok Min, MD, PhD, Jin-Young Min, PhD 

Kyoung-Bok Min, MD, PhD, Jin-Young Min, PhD 
Correlation of allergen-specific T follicular helper cell counts with specific IgE levels and efficacy of allergen immunotherapy  Yin Yao, MD, Cai-Ling.

Correlation of allergen-specific T follicular helper cell counts with specific IgE levels and efficacy of allergen immunotherapy  Yin Yao, MD, Cai-Ling.
The effects of upper respiratory infection on T-cell proliferation and steroid sensitivity of asthmatics  Elcio O. Vianna, MD, PhD, Jay Westcott, PhD,

The effects of upper respiratory infection on T-cell proliferation and steroid sensitivity of asthmatics  Elcio O. Vianna, MD, PhD, Jay Westcott, PhD,
IL-4 induces IL-13–independent allergic airway inflammation

IL-4 induces IL-13–independent allergic airway inflammation
IgE cross-linking impairs monocyte antiviral responses and inhibits influenza-driven TH1 differentiation  Regina K. Rowe, MD, PhD, David M. Pyle, MD,

IgE cross-linking impairs monocyte antiviral responses and inhibits influenza-driven TH1 differentiation  Regina K. Rowe, MD, PhD, David M. Pyle, MD,
IL-25 and CD4+ TH2 cells enhance type 2 innate lymphoid cell–derived IL-13 production, which promotes IgE-mediated experimental food allergy  Jee-Boong.

IL-25 and CD4+ TH2 cells enhance type 2 innate lymphoid cell–derived IL-13 production, which promotes IgE-mediated experimental food allergy  Jee-Boong.
Ann-Marie M. Schoos, MD, PhD, Jacob D

Ann-Marie M. Schoos, MD, PhD, Jacob D
Allergy prevention starts before conception: Maternofetal transfer of tolerance protects against the development of asthma  Tobias Polte, PhD, Christian.

Allergy prevention starts before conception: Maternofetal transfer of tolerance protects against the development of asthma  Tobias Polte, PhD, Christian.
Increased expression of nuclear factor of activated T cells 1 drives IL-9–mediated allergic asthma  Sonja Koch, PhD, Anna Graser, PhD, Hooman Mirzakhani,

Increased expression of nuclear factor of activated T cells 1 drives IL-9–mediated allergic asthma  Sonja Koch, PhD, Anna Graser, PhD, Hooman Mirzakhani,
Expression of IL-4 receptor α on smooth muscle cells is not necessary for development of experimental allergic asthma  Frank Kirstein, PhD, William G.C.

Expression of IL-4 receptor α on smooth muscle cells is not necessary for development of experimental allergic asthma  Frank Kirstein, PhD, William G.C.
Frank Kirstein, PhD, Natalie E

Frank Kirstein, PhD, Natalie E
Protective role of nuclear factor of activated T cells 2 in CD8+ long-lived memory T cells in an allergy model  Roman Karwot, PhD, Joachim H. Maxeiner,

Protective role of nuclear factor of activated T cells 2 in CD8+ long-lived memory T cells in an allergy model  Roman Karwot, PhD, Joachim H. Maxeiner,
Stephen J. Fowler, MD, Gaël Tavernier, PhD, Robert Niven, MD 

Stephen J. Fowler, MD, Gaël Tavernier, PhD, Robert Niven, MD 
Ramses Ilarraza, PhD, Yingqi Wu, Christopher D

Ramses Ilarraza, PhD, Yingqi Wu, Christopher D
Anti–IL-5 (mepolizumab) therapy reduces eosinophil activation ex vivo and increases IL- 5 and IL-5 receptor levels  Miguel L. Stein, MD, Joyce M. Villanueva,

Anti–IL-5 (mepolizumab) therapy reduces eosinophil activation ex vivo and increases IL- 5 and IL-5 receptor levels  Miguel L. Stein, MD, Joyce M. Villanueva,
Targeting Toll-like receptors on dendritic cells modifies the TH2 response to peanut allergens in vitro  Pierre Pochard, PhD, Brian Vickery, MD, M. Cecilia.

Targeting Toll-like receptors on dendritic cells modifies the TH2 response to peanut allergens in vitro  Pierre Pochard, PhD, Brian Vickery, MD, M. Cecilia.
Restoration of T-box–containing protein expressed in T cells protects against allergen- induced asthma  Jung Won Park, MD, Hyun Jung Min, MS, Jung Ho Sohn,

Restoration of T-box–containing protein expressed in T cells protects against allergen- induced asthma  Jung Won Park, MD, Hyun Jung Min, MS, Jung Ho Sohn,
Group 2 innate lymphoid cells facilitate sensitization to local, but not systemic, TH2- inducing allergen exposures  Matthew J. Gold, BSc, Frann Antignano,

Group 2 innate lymphoid cells facilitate sensitization to local, but not systemic, TH2- inducing allergen exposures  Matthew J. Gold, BSc, Frann Antignano,
Antigen-specific effector CD8 T cells regulate allergic responses via IFN-γ and dendritic cell function  Yafang Tang, BSc, Shou Ping Guan, BSc, Benson.

Antigen-specific effector CD8 T cells regulate allergic responses via IFN-γ and dendritic cell function  Yafang Tang, BSc, Shou Ping Guan, BSc, Benson.
Dorothy M. Ryan, MD, Stephen J. Fowler, MD, Robert M. Niven, MD 

Dorothy M. Ryan, MD, Stephen J. Fowler, MD, Robert M. Niven, MD 

Similar presentations

Ads by Google