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Modulation of circulating cell–endothelial cell interaction by erythropoietin in lean and obese mice with cecal ligation and puncture  Vidula Vachharajani,

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Presentation on theme: "Modulation of circulating cell–endothelial cell interaction by erythropoietin in lean and obese mice with cecal ligation and puncture  Vidula Vachharajani,"— Presentation transcript:

1 Modulation of circulating cell–endothelial cell interaction by erythropoietin in lean and obese mice with cecal ligation and puncture  Vidula Vachharajani, Shantel Vital, Janice Russell  Pathophysiology  Volume 17, Issue 1, Pages 9-18 (February 2010) DOI: /j.pathophys Copyright © 2009 Elsevier Ireland Ltd Terms and Conditions

2 Fig. 1 Effects of erythropoietin (EPO) treatment on the leukocyte adhesion responses elicited by cecal ligation and puncture (CLP) in lean (WT) and obese (ob/ob) mice. p<0.05: (*) vs. SHAM, (†) vs. CLP, (‡) vs. WT CLP. Pathophysiology  , 9-18DOI: ( /j.pathophys ) Copyright © 2009 Elsevier Ireland Ltd Terms and Conditions

3 Fig. 2 Effects of erythropoietin (EPO) treatment on the platelet adhesion responses elicited by cecal ligation and puncture (CLP) in lean (WT) and obese (ob/ob) mice. p<0.05: (*) vs. SHAM, (†) vs. CLP, (‡) vs. WT CLP. Pathophysiology  , 9-18DOI: ( /j.pathophys ) Copyright © 2009 Elsevier Ireland Ltd Terms and Conditions

4 Fig. 3 Effect of erythropoietin on Evans Blue (EB) leakage in the brain tissue elicited by SHAM and cecal ligation and puncture (CLP) in WT and ob/ob mice. p<0.05: (*) vs. SHAM, (†) vs. CLP, (‡) vs. WT CLP. Pathophysiology  , 9-18DOI: ( /j.pathophys ) Copyright © 2009 Elsevier Ireland Ltd Terms and Conditions

5 Fig. 4 Effects of erythropoietin (EPO) on locomotor activity (sickness behavior) of lean (WT) and obese (ob/ob) mice after SHAM and cecal ligation and puncture (CLP). p<0.05: (*) vs. SHAM, (†) vs. CLP, (#) vs. WT SHAM+EPO, (‡) vs. WT CLP, (κ) vs. WT CLP+EPO. Pathophysiology  , 9-18DOI: ( /j.pathophys ) Copyright © 2009 Elsevier Ireland Ltd Terms and Conditions

6 Fig. 5 Serum tumor necrosis factor (TNF) levels WT and ob/ob mice subjected to SHAM and CLP for 4h. Levels are expressed as pg/ml serum. p<0.05: (*) vs. SHAM, (†) vs. CLP, (‡) vs. WT CLP. Pathophysiology  , 9-18DOI: ( /j.pathophys ) Copyright © 2009 Elsevier Ireland Ltd Terms and Conditions

7 Fig. 6 Leukocyte and platelet adhesion in sham, CLP, CLP+EPO and CLP+TNF mAb treated ob/ob mice. Please note the down regulation of both LA and PA in mice treated with EPO and TNF mAb to the same extent. p<0.05: (*) vs. SHAM, (†) vs. CLP. Pathophysiology  , 9-18DOI: ( /j.pathophys ) Copyright © 2009 Elsevier Ireland Ltd Terms and Conditions

8 Fig. 7 P-selectin expression in the brain of ob/ob mice subjected to sham, CLP, CLP+EPO and CLP+TNF mAb. Please note the down regulation of P-selectin expression in CLP+EPO and CLP+TNF mAb mice was significantly lower compared to CLP, and to the same extent of each other. p<0.05: (*) vs. SHAM, (†) vs. CLP. Pathophysiology  , 9-18DOI: ( /j.pathophys ) Copyright © 2009 Elsevier Ireland Ltd Terms and Conditions

9 Fig. 8 Evans Blue leakage in the brain of the ob/ob mice subjected to sham, CLP, CLP+EPO, CLP+TNF mAb and CLP+P-selectin mAb. Please note significant attenuation of EB leakage in all treated groups was to the same degree compared to untreated CLP. p<0.05: (*) vs. SHAM, (†) vs. CLP. Pathophysiology  , 9-18DOI: ( /j.pathophys ) Copyright © 2009 Elsevier Ireland Ltd Terms and Conditions


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